1. A B D C E F Figure 1 + + + + + + - - + + - - - - - - + + - + - + - +
FLAG
β-actin
p53
SET9
150 50 40
M.w.
kDa D
β-actin
p53
G9a 50 40
150
M.w.
kDa C E F
P21 expression
H1299 (p53 -/-) cells
p53
F-hG9a
F-mG9a
Normalized relative band intensity
M.w.
kDa
Dox
p21 20
Puma 20
M.w.
kDa
mG9a
Puma expression
hG9a
150
150
G9a
p53 50
p53 50
Normalized relative band intensity
b-actin
β-actin 40 40
Figure 1

2. mRNA Expression (fold)C
mRNA Expression (fold)
pLKO
M.w.
kDa
shG9a
150
G9a
β-actin 40
shG9a#pool
shG9a#2 D
mRNA Expression (fold) B
F-hG9a(S)
F-hG9a(L)
F-hGLP
M.w.
kDa
Dox
FLAG
p53
β-actin
150 50 40
Figure 2

3. A B Figure 3 p53 β-actin Fold activation, PUMA-luc
HCT116 (p53+/+) cells
treated with BIX-01294
Fold activation, PUMA-luc
M.w.
kDa 50 40
Figure 3

4. Dual cross-linking ChIP: HCT116 cells (α-CBP)
Relative Fold Change
H1299 (p53 -/-) Cells
DAPI (DNA)
GFP (p53)
Flag (G9a)
merge
H1299 (p53 -/-) cells
p21 promoter
Start Site
IP: α-FLAG
M.w.
kDa
INP
α-IgG
α-FLAG
p53RE
CBP
-2250 +1
250
150
Flag-G9a (S) 50
p53
H460 (p53 +/+) cells
PUMA promoter
Start Site
CBP
G9a (S)
p53RE
DAPI (DNA)
CBP
-224 +1
Flag (G9a)
merge
p53 E
Dual cross-linking ChIP: HCT116 cells (α-CBP) G H F
M.w.
kDa
250
150 50 40
M.w.
kDa
CBP
250
CBP
150
FLAG-G9a
G9a
β-actin
p53 40
β-actin
Figure 4

5. A B -Dox D C Figure 5 + - - + + + + + mG9a pCDNA3 hG9a(S)
H460 (p53 +/+) cells
-Dox
+Dox D
H1299 (p53 -/-) cells
pLKO C
pLKO-shG9a
M.w.
kDa
p53
Dox
G9a
150
PARP
100
Cleaved-PARP
p53 50
β-actin 40
Figure 5

6. A B Figure 6 GEO dataset ID: GSE31210 GEO dataset ID: GSE13213
GEO dataset Type: lung adenocarcinoma I-II stage
group=1: low expression of G9A
group=2: high expression of G9A
N Observed Expected (O-E)^2/E (O-E)^2/V
group=
group=
Chisq= 3.3 on 1 degrees of freedom, p=
GEO dataset ID: GSE13213
GEO dataset Type: lung cancer A B
Survival differences between Group=1 and Group=2
Group=1: green , strong positive correlation (0.79) between TP53 and G9A
Group=2: red , no (or negative) correlation (-0.22) between TP53 and G9A
N Observed Expected (O-E)^2/E (O-E)^2/V
group=
group=
Chisq= 9.5 on 1 degrees of freedom, p=
Figure 6

7. p53 p53 Transcription NO transcription PCAF CBP Ac Ac Human G9a
CDP/Cut, Gfi1, UHRF1, WIZ
PCAF
CBP
hG9a
CBP
mG9a Ac Ac Me
p53
p53 Me
TATA
TATA
Transcription
NO transcription
Human G9a
Mouse G9a
Figure 7

8. Supplementary S1 + + + + Coomassie [H ]-Me M.w. kDa GST-p53300-393
Histones
6His-G9a
130
100 70
GST-p 55 35 25
Histones 15
KMT G9a 95
130 72
6His-Set7/9 55
GST-p 43 34 26
Histones 17
KMT Set7/9
130 95 72
Flag-Smyd2 55 43
GST-p 34 26
Histones 17
Coomassie
[H ]-Me 3
KMT Smyd2
Supplementary S1

9. M.w.
kDa
G9a
150
mp53 50
β-actin 40
Supplementary S2

10. M.w.
kDa
β-actin
p53
Flag-G9a
150 50 40
Supplementary S3

11. Relative Fold Change
Supplementary S4

12. Supplementary S5 - + - - - + + + + H460 (p53 +/+) cells hG9a(S) M.w.
hG9a(S)
M.w.
kDa
mG9a
Dox
PARP
100
Cleaved-PARP
G9a
150
p53 50
β-actin 40
Supplementary S5

13. Supplementary S6 GEO dataset ID: GSE36471
GEO dataset Type: lung cancer
Survival differences between Group=1 and Group=2
Group=1: green , strong positive correlation (0.67) between TP53 and G9A
Group=2: red , no (or negative) correlation (-0.64) between TP53 and G9A
N Observed Expected (O-E)^2/E (O-E)^2/V
group=
group=
Chisq= 6.2 on 1 degrees of freedom, p= 0.013
Supplementary S6

14. Supplementary S7 GEO dataset ID: GSE39582 Cancer Type: Colon cancer
p-value:
Positive effect: EHMT2__high|TP53__high
N Observed Expected (O-E)^2/E (O-E)^2/V
EHMT2__high|TP53__high;
Other;
Chisq= 5.2 on 1 degrees of freedom, p=
Supplementary S7