1. MFS1 expression in IPO323 MFS1 replacement mutants.
MFS1 expression in IPO323 MFS1 replacement mutants. MFS1 expression was measured by qRT-PCR relative to three reference genes (β-tubulin, eF1a, and actin). Median values (n = 4 to 8) and interquartile range are indicated in the plot. Transformants are indicated by the symbol #. The letters in parentheses refer to the recombination event in Fig. 4. A type a recombination leads to the promoter insert of 519 bp and the MDR phenotype, while a type b recombination event does not. #4T corresponds to expression data from four independent transformants with a type b recombination event (n = 8). ***, significant difference of the MFS1 expression levels between strains or groups of strains according to Kruskall and Wallis’ nonparametric statistical test with a risk threshold at 0.1%.
Selim Omrane et al. mSphere 2017; doi: /mSphere