1. Transcriptional dynamics during lineage commitment
Transcriptional dynamics during lineage commitment Heatmap exhibiting transcriptional dynamics of nine distinct gene modules during fate transitions. Data represent scaled gene expression for the 963 micro‐clusters, which are grouped by branch annotation, and ordered by increasing developmental progression (distance from the MST root—micro‐cluster with the highest AVP expression; Materials and Methods). The top 20 markers for each cluster are shown; the module labels for all 517 “branch‐dependent” genes are listed in (Table EV3).Mean expression for each module, displayed across four trajectories as a function of normalized developmental progression (Materials and Methods), connecting HSC/MPP to each of four downstream lineages.Biological GO term enrichments for genes in each transcriptional module.We observe enrichments for PU.1 (ETS) or GATA2 motifs in the upstream regions of all nine gene modules. Enrichments correspond to enrichment scores from i‐cisTarget (Imrichová et al, 2015; Herrmann et al, 2012).Transcriptional dynamics of GATA and ETS motif binding transcription factors across all four trajectories, using the same color scheme as (B). A local polynomial regression with span = 0.9 was used for smoothing, with the underlying gene expression data shown as individual points. Additional genes are shown in Fig EV3A.
Shiwei Zheng et al. Mol Syst Biol 2018;14:e8041
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