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In vitro migratory capacity of CD34+ cells is related to hematopoietic recovery after autologous stem cell transplantation by Carlijn Voermans, Marisha.

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Presentation on theme: "In vitro migratory capacity of CD34+ cells is related to hematopoietic recovery after autologous stem cell transplantation by Carlijn Voermans, Marisha."— Presentation transcript:

1 In vitro migratory capacity of CD34+ cells is related to hematopoietic recovery after autologous stem cell transplantation by Carlijn Voermans, Marisha L. K. Kooi, Sjoerd Rodenhuis, Hans van der Lelie, C. Ellen van der Schoot, and Winald R. Gerritsen Blood Volume 97(3): February 1, 2001 ©2001 by American Society of Hematology

2 PB CD34+ cells obtained from mobilized patients show reduced spontaneous and SDF-1–induced migration across fibronectin-coated filters compared to PB CD34+ cells of healthy mobilized donors.Spontaneous and SDF-1–induced migration (100 ng/mL and 300 ng/mL) o... PB CD34+ cells obtained from mobilized patients show reduced spontaneous and SDF-1–induced migration across fibronectin-coated filters compared to PB CD34+ cells of healthy mobilized donors.Spontaneous and SDF-1–induced migration (100 ng/mL and 300 ng/mL) of PB-derived CD34+ cells after 4 hours of 6 healthy mobilized donors (■), 18 patients with nonhematologic malignancies (▨), and 10 patients with hematologic malignancies (▪). Spontaneous migration and SDF-1–induced migration (100 ng/mL and 300 ng/mL SDF-1) of PB CD34+ cells derived from healthy donors was significantly higher than from patients with nonhematologic or hematologic malignancies (* = P < .05; ** = P < .01; *** = P < .001). No significant differences in spontaneous and SDF-1–induced migration were observed between the 2 patient groups. Each bar represents the mean ± SEM. Carlijn Voermans et al. Blood 2001;97: ©2001 by American Society of Hematology

3 Migratory capacity of patient-derived PB CD34+ cells
Migratory capacity of patient-derived PB CD34+ cells.The migratory capacity of patient-derived PB CD34+ cells was determined by subtracting the percentage of migration induced by 100 ng/mL SDF-1 from the percentage of migration induced by 300 ng/mL SDF-1. Migratory capacity of patient-derived PB CD34+ cells.The migratory capacity of patient-derived PB CD34+ cells was determined by subtracting the percentage of migration induced by 100 ng/mL SDF-1 from the percentage of migration induced by 300 ng/mL SDF-1. A positive correlation (r = 0.5;P < .01) was observed between the increment in migration and the percentage migration induced by 100 ng/mL SDF-1 for a group of 27 patients. Carlijn Voermans et al. Blood 2001;97: ©2001 by American Society of Hematology

4 Negative correlation between spontaneous migration in vitro and neutrophil recovery in vivo in patients undergoing autologous PBSCT.On the y-axis is depicted the day on which the neutrophil count in the patients in circulation reached 0.1 × 109 cells/L (day... Negative correlation between spontaneous migration in vitro and neutrophil recovery in vivo in patients undergoing autologous PBSCT.On the y-axis is depicted the day on which the neutrophil count in the patients in circulation reached 0.1 × 109 cells/L (day gran 100). The x-axis represents the spontaneous migration (percentage input). A negative correlation (r = −0.5; P < .05) was found between spontaneous migration in vitro and neutrophil recovery in vivo for a group of 22 patients. Carlijn Voermans et al. Blood 2001;97: ©2001 by American Society of Hematology

5 Negative correlation between SDF-1–induced migration in vitro and neutrophil recovery in vivo in patients undergoing autologous PBSCT.On the y-axis is depicted the day on which the neutrophil count in the patients in circulation reached 0.1 × 109 cells/L (d... Negative correlation between SDF-1–induced migration in vitro and neutrophil recovery in vivo in patients undergoing autologous PBSCT.On the y-axis is depicted the day on which the neutrophil count in the patients in circulation reached 0.1 × 109 cells/L (day gran 100). The x-axis represents the SDF-1–induced migration (percentage input). A negative correlation (r = −0.5; P < .05) was found between SDF-1–induced (100 ng/mL) migration in vitro and neutrophil recovery in vivo for a group of 21 patients. Carlijn Voermans et al. Blood 2001;97: ©2001 by American Society of Hematology

6 Correlation between CXCR-4 expression and mobilization of CD34+ cells per kilogram body weight during the first course of leukapheresis.An exponential decrease of mobilized CD34+ cells is observed when the MFI of CXCR-4 on the CD34+ cells is higher. Correlation between CXCR-4 expression and mobilization of CD34+ cells per kilogram body weight during the first course of leukapheresis.An exponential decrease of mobilized CD34+ cells is observed when the MFI of CXCR-4 on the CD34+ cells is higher. Linear regression analysis was performed with the natural logarithm (Ln) of the number of CD34+ cells as a dependent variable and MFI of CXCR-4 as an independent variable. The following function describes this model: CD34 = 18.062xe(−0.033 × (CXCR-4)). The coefficient of CXCR-4 is significantly different from 0 (P < .0001). n = 54. Carlijn Voermans et al. Blood 2001;97: ©2001 by American Society of Hematology

7 CXCR-4 expression on PB CD34+ cells during mobilization in 8 patients undergoing autologous PBSCT.On the y-axis the CXCR-4 expression in MFI of all CD34+cells is given after correction for the PE-labeled IgG2a isotype control. CXCR-4 expression on PB CD34+ cells during mobilization in 8 patients undergoing autologous PBSCT.On the y-axis the CXCR-4 expression in MFI of all CD34+cells is given after correction for the PE-labeled IgG2a isotype control. On the x-axis, the day of leukapheresis is depicted. Carlijn Voermans et al. Blood 2001;97: ©2001 by American Society of Hematology

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