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Published byKvěta Vávrová Modified over 6 years ago
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High Resolution Melting Analysis for JAK2 Exon 14 and Exon 12 Mutations
Inmaculada Rapado, Silvia Grande, Enriqueta Albizua, Rosa Ayala, José-Angel Hernández, Miguel Gallardo, Florinda Gilsanz, Joaquin Martinez-Lopez The Journal of Molecular Diagnostics Volume 11, Issue 2, Pages (March 2009) DOI: /jmoldx Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions
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Figure 1 Normalized temperature-shifted difference graph showing mutation detection for JAK2 exons 12 and 14. The melting profile of a wild-type control has been chosen as a horizontal baseline. A: A difference graph of JAK2 V617F. Mutated samples with high and low allele burden and wild-type samples are shown. B: The difference graph of JAK2 exon 12 shows wild-type samples and three different mutations: A, B and C. Curve A represents the duplication and curves B and C represent the two deletions. The Journal of Molecular Diagnostics , DOI: ( /jmoldx ) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions
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