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Evidence for markers of hypoxia and apoptosis in explanted human carotid atherosclerotic plaques  Alessandra Bitto, MD, Giovanni De Caridi, MD, Francesca.

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Presentation on theme: "Evidence for markers of hypoxia and apoptosis in explanted human carotid atherosclerotic plaques  Alessandra Bitto, MD, Giovanni De Caridi, MD, Francesca."— Presentation transcript:

1 Evidence for markers of hypoxia and apoptosis in explanted human carotid atherosclerotic plaques 
Alessandra Bitto, MD, Giovanni De Caridi, MD, Francesca Polito, PhD, Margherita Calò, PhD, Natasha Irrera, JD, Domenica Altavilla, PhD, Francesco Spinelli, MD, Francesco Squadrito, MD  Journal of Vascular Surgery  Volume 52, Issue 4, Pages (October 2010) DOI: /j.jvs Copyright © 2010 Society for Vascular Surgery Terms and Conditions

2 Fig 1 A, Macroscopic appearance of a normal carotid artery. Arrow head indicates an early fatty streak. B, H&E staining used for routine tissue preparation, nuclei stain blue, while connective tissue stains pink. The rectangle represents the area studied in C and D. Original magnification ×2. C, H&E staining, detail showing a normal arterial morphology. Original magnification ×20. D, Masson's trichrome staining, collagen fibers stain green, while muscle fibers stain red. Arrowhead indicates muscular fibers, green color indicates collagen tissue. Original magnification ×20. E, Macroscopic appearance of an atheromatous common carotid artery. F, H&E staining. The rectangle represents the area studied in H. Original magnification ×2. G, Masson's trichrome staining. The rectangle represents the area studied in I. Original magnification ×2. H, H&E staining, detail showing an altered morphological architecture with inflammatory infiltrate. Arrow head indicates adipose tissue deposition. Original magnification ×20. I, Masson's trichrome staining, arrowheads indicates calcification, and green color indicates fibrose tissue. Original magnification ×20. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

3 Fig 2 A, Representative Western blot of p-eNOS in carotid arteries. Bars represents mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. B, Representative Western blot of p-eNOS in carotid arteries. Bars represents mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. C, Representative Western blot of p-ERK in carotid arteries. Bars represents mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. D, Representative Western blot of p-ASK-1 in carotid arteries. Bars represents mean ± SD. Controls n = 5. All the other groups n=10. *P < .05 vs controls. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

4 Fig 3 A, HIF-1α immunostaining in fibrocalcific carotid artery. Arrow heads shows nuclear reactivity. Original magnification ×40. P < .01 vs controls. B, p-JNK immunostaining in fibrocalcific carotid artery. Arrow heads shows diffuse cytoplasmic reactivity. Original magnification ×40. P < .01 vs controls. C, Representative Western blot of HIF-1α in carotid arteries. Bars represent mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. D, Representative Western blot of p-JNK in carotid arteries. Bars represents mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

5 Fig 4 A, Representative Western blot of p-p38 in carotid arteries. Bars represent mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. B, Representative Western blot of active caspase-9 in carotid arteries. Bars represent mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. C, Representative Western blot of OPG in carotid arteries. Bars represents mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. D, Representative Western blot of RANKL in carotid arteries. Bars represent mean ± SD. Controls n = 5. All the other groups n = 10. *P < .05 vs controls. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Proposed cascade of events triggered by oxidative stress in atherosclerotic plaque. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

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