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Isolation and Identification of Spinach Pigments

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Presentation on theme: "Isolation and Identification of Spinach Pigments"— Presentation transcript:

1 Isolation and Identification of Spinach Pigments
Professor Matt And Colleen

2 Photosynthesis Plants and organisms use for food Requires:
Carbon Dioxide Water Sunlight Highly conjugated pigments Metal ions

3 Pigments Highly conjugated proteins 4 types:
Chlorophyll a Chlorophyll b B-carotene Xanthrophylls violaxanthin Lutein Structures provided in lab manual

4 Chromatography “color graphing” Separation using intermolecular forces
Inter = between molecules Capillary action IMF strength due to: Type Shape Size

5 Rf value Calculation using your measurements
Distance moved by a compound, mm Distance moved by the solvent, mm

6 Polarity Based on: Electronegativity Molecular weight
Shape/orientation of molecules

7 Groups for Absorbency Group 1: 400-430, 600-640

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