1. The zinc finger domain of scPan3 binds polyA RNA Overlay of the 20 best NMR models of the CCCH zinc finger of Saccharomyces cerevisiae Pan3 (residues 1–41) showing the protein backbone colored in rainbow from N‐ to C‐terminus.
The zinc finger domain of scPan3 binds polyA RNA Overlay of the 20 best NMR models of the CCCH zinc finger of Saccharomyces cerevisiae Pan3 (residues 1–41) showing the protein backbone colored in rainbow from N‐ to C‐terminus. Cartoon representation of the zinc finger of scPan3 with a modeled zinc ion (red sphere). The disordered termini have been omitted. Close‐up of the zinc finger of scPan3 (ribbon) showing functional residues in stick representation. The Zn atom (red sphere) is coordinated to His34 and the three cysteines. The scPan3 zinc finger binds polyA RNA. Plot of the magnitude of chemical shift perturbation of each residue, calculated from 15N‐1H HSQC spectra of 150 μM scPan3 zinc finger after addition of 150 μM 15‐mer polyA RNA. Many of the zinc finger residues experience large changes in their chemical environment in the presence of polyA RNA. Residues discussed in the text are labeled. The peak for Arg15 (red) disappears upon RNA binding. (Also see Supplementary Fig S4).
Jana Wolf et al. EMBO J. 2014;33:
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