1. Coral Bleaching Experiment
By Group 1:
L.C.
Yao
Leo
Marcel

2. Procedure Week 3 Determine Zooxanthelle Density
Measure Chlorophyll Concentration
Determine Protein Concentration

3. Coral Bleaching
Dispelling of symbiotic zooxanthelle by coral host due to environmental stress
Affected by temperature, salinity, amount of UVA radiation, etc

4. Aiptasia Numbers Mass of Aiptasia = .1396 grams
Total Zooxs = 6.4 x 10^6 zoox/mL
Zook Density = 92.0 x 10^6 zoox/g wet weight
Protein Concent = 1.70 mg/g
Chlorophyll ng/10^6 cells =
47.62 ng/10^6 cell

5. Amount of Chlorophyll In Aiptasia In Xenia Wavelength Absorbance
665nm →
647nm →
630nm →
In Xenia
Wavelength Absorbance
665nm →
647nm →
630nm →

6. Protein Content Aiptasia Xenia Abs(avg) .4895 .743 1.055 1.268 1.480
.1mL of .25 mg/mL BSA + 5mL Bradford
.1mL of .50mg/mL BSA + 5mL Bradford
.1mL of .75 mg/mL BSA + 5mL Bradford
.1mL of 1.00 mg/mL BSA + 5mL Bradford
.1mL of 1.25 mg/mL BSA + 5mL Bradford
.1 ml of homogenate+ 5mL of Bradford
Abs(avg)
.4895
.743
1.055
1.268
1.480
2.171
Xenia
.1mL of .25 mg/mL BSA + 5mL Bradford
.1mL of .50mg/mL BSA + 5mL Bradford
.1mL of .75 mg/mL BSA + 5mL Bradford
.1mL of 1.00 mg/mL BSA + 5mL Bradford
.1mL of 1.25 mg/mL BSA + 5mL Bradford
.1 ml of homogenate+ 5mL of Bradford
Abs(avg)
.385
.641
.900
1.116
1.284
.074

7. Graph

8. Calculations Protein: Chlorophyll: Chlorophyll ng/10^6 cells:
2.171 = X
= mg/g
Chlorophyll:
Ca = (11.85A665)-(1.54A647)-(0.08A630)
= ug/mL
Chlorophyll ng/10^6 cells:
Ug chl / zoox = (.4876 ug chl / 6.4 x 10^6) =
mL mL mL mL
ug chl / 1.6cm = ng 10^6 cells

9. Group One’s Coral Bleaching Experiment
Coral Experiment
Group One’s Coral Bleaching Experiment
Hypothesis:
Coral will expel zooxs due to an increase in tank temperature
Control Tank:
Create a tank that remains at a constant temperature, salinity, UVA exposure, etc.
Conclusion:
Determine if in fact zooxs are expelled because of temperature
Each Week:
Determine number of zooxs, test cholorphyll and protein, compare with control tank
Variable Tank:
In this tank, increase the temperature by 3 degrees Celsius and leave at new temp for remaining 3 weeks

10. THE END!!!