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Getting Things Backwards to Prevent Primer Dimers

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Presentation on theme: "Getting Things Backwards to Prevent Primer Dimers"— Presentation transcript:

1 Getting Things Backwards to Prevent Primer Dimers
Mark A. Poritz, Kirk M. Ririe  The Journal of Molecular Diagnostics  Volume 16, Issue 2, Pages (March 2014) DOI: /j.jmoldx Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Cooperative primers amplify template, but not primer dimers. A and B: Cooperative primers (red lines and green lines) are shown during the PCR amplification of a template (yellow lines and blue lines). The two forms of cooperative primers consist of two DNA sequences joined together either 5′ to 5′ (A) or 5′ to 3′ (B) through a polyethylene glycol linker (shown as a dotted arc).3 The 3′ ends of the cooperative primers are indicated by arrowheads. The vertical line on the 3′ end of the capture sequence (A) indicates that it is chemically blocked from being extended. The initiation (I) and propagation (P) steps for primer dimers are shown for cooperative (C) and conventional (D) primers. Satterfield's3 data indicate that cooperative primers block the propagation step for primer dimers (C, red cross).3 For brevity, only the bottom strand is included in the DNA synthesis steps depicted here. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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