1. Inhibition of ephrinB2/EphB4 signaling does not prevent pericyte recruitment
Inhibition of ephrinB2/EphB4 signaling does not prevent pericyte recruitment Muscles were harvested 3 and 4 days after implantation of V‐low or V‐low sEphB4 myoblast clones. Immunofluorescence staining of endothelium (CD31, red), pericytes (NG2, green), smooth muscle cells (α‐SMA, cyan), and nuclei (DAPI, blue). In both conditions, initial vascular enlargements after 3 and 4 days were tightly associated with mural cells displaying a pericyte morphology, which, however, upregulated α‐SMA expression in the presence of EphB4 blockade. *lumen of vascular enlargements; scale bar = 25 μm.Co‐staining for laminin (LAM, red) confirmed the pericyte identity of both α‐SMA‐positive and α‐SMA‐negative mural cells, as both were embedded inside the endothelial basal lamina. *lumen of vascular enlargements; scale bar = 25 μm.Gene expression of Pdgfb and Pdgfrb was quantified in skeletal muscles 3 days after myoblast implantation and expressed as fold‐change vs. control muscles. Mean ± SEM; n = 4 independent samples/group; ***P < 0.001, ###P < 0.001 vs. Ctrl (*) or vs. sEphB4 (#) (one‐way ANOVA with Bonferroni multiple comparisons test, after data normalization by logarithmic‐transformation).
Elena Groppa et al. EMBO Rep. 2018;embr
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