1. Fragmentation of embryos is associated with both necrosis and apoptosis 
Hee-Jun Chi, Ph.D., Jung-Jin Koo, M.D., Soon-Young Choi, M.Sc., Hyeon-Jeong Jeong, M.D., Sung-Il Roh, M.D. 
Fertility and Sterility 
Volume 96, Issue 1, Pages (July 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
DNA integrity of unfragmented (A, B) and fragmented (C, D) human embryos, as assessed by the comet assay. Magnification, A, C: × 400; B, D: × 200.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
TEMs of human fragmented embryos. The normal blastomere contains a nucleus and a high concentration of mitochondria, but the anucleate fragments contain a low concentration of mitochondria (ATP depletion). Magnification, A: ×1,570, B: ×5,520.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Beneficial effect of fragment removal on the subsequent development of neighboring blastomeres. (A) Human embryos before fragment removal (day 2), (B) after fragment removal (day 2), and (C) after culture for 24 hours (day 3). Magnification, ×200.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Annexin V and PI staining of mouse and human fragmented embryos. (A) Unfragmented mouse embryo, (B) negative for annexin and PI. (C, E, G) Fragmented mouse embryos, (D, F) positive for PI (necrosis), (H) positive for annexin and PI, membrane bleb (weak green staining, apoptosis). (I) Good-quality human embryo, (J) negative for annexin V and PI. (K) Fragmented human embryo, (L) weakly positive for PI. (M) Severely fragmented and degenerating human embryo, (N) strongly positive for PI. (O) Fragmented and arrested human embryo, (P) positive for both annexin V and PI. Magnification, ×400.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions