Presentation is loading. Please wait.

Presentation is loading. Please wait.

Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana,

Published byRegina Jansen Modified over 6 years ago

Similar presentations

Presentation on theme: "Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana,"— Presentation transcript:

1 Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana, L Seoke, J Hyera, G Matlho, F Vandenbussche, K De Clercq

2 The Study Can unbiased RNASeq produce complete FMDV genome sequences directly from clinical samples? 3 strong positive epithelial samples (CT range 14,63 – 16,18) from Zambia and Namibia

3 Results Unbiased RNASeq produces complete genomes with high average coverage in 2/3 samples limited gap closure (Sanger sequencing) needed in 3rd sample due to low coverage (limited % of FMDV reads). Potential for unbiased detection of co-infections If only targeting FMDV, targeted approaches may produce better coverage. e.g. Logan G, Freimanis GL, King DJ, Valdazo-Gonzalez B, Bachanek-Bankowska K, Sanderson ND, Knowles NJ, King DP, Cottam EM. (2014) BMC Genomics 15:828

4 Conclusion These data demonstrate the feasibility of direct sequencing of complete FMDV coding sequences from samples from symptomatic animals The complete genome sequences of O/ZAM14/2010, SAT1/NAM01/2010 and SAT2/ZAM18/2009 were obtained

Download ppt "Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana,"

Similar presentations

Hepatitis B Virus. Hepatitis B - Clinical Features Incubation period:Average 60-90 days Range 45-180 days Clinical illness (jaundice):

Hepatitis B Virus. Hepatitis B - Clinical Features Incubation period:Average days Range days Clinical illness (jaundice):
Virus discovery-454 sequencing

Virus discovery-454 sequencing
WRLFMD Identification of a Ninth Foot-and-Mouth Disease Virus Type O Topotype and Evidence for a Recombination Event in its Evolution Nick J. Knowles,

WRLFMD Identification of a Ninth Foot-and-Mouth Disease Virus Type O Topotype and Evidence for a Recombination Event in its Evolution Nick J. Knowles,
1/03/09 De 89 à 98. 1/03/09 De 89 à 98 1/03/09 De 89 à 98.

1/03/09 De 89 à 98. 1/03/09 De 89 à 98 1/03/09 De 89 à 98.
Transcriptomics Breakout. Topics Discussed Transcriptomics Applications and Challenges For Each Systems Biology Project –Host and Pathogen Bacteria Viruses.

Transcriptomics Breakout. Topics Discussed Transcriptomics Applications and Challenges For Each Systems Biology Project –Host and Pathogen Bacteria Viruses.
High Throughput Sequencing

High Throughput Sequencing
Laboratory Diagnosis of Virus Infections

Laboratory Diagnosis of Virus Infections
Next Generation Sequencing and its data analysis challenges Background Alignment and Assembly Applications Genome Epigenome Transcriptome.

Next Generation Sequencing and its data analysis challenges Background Alignment and Assembly Applications Genome Epigenome Transcriptome.
B19 inactivation by heat treatment with epithelial cell lines Yoshiaki Okada Kiyoko Umemori Kazunari Yamaguchi National Institute of Infectious Disease.

B19 inactivation by heat treatment with epithelial cell lines Yoshiaki Okada Kiyoko Umemori Kazunari Yamaguchi National Institute of Infectious Disease.
Molecular biology and evolution in genomics era Li Jun and Frederick C.C. Leung 5N01, School of Biological Science, The University.

Molecular biology and evolution in genomics era Li Jun and Frederick C.C. Leung 5N01, School of Biological Science, The University.
***. The Air Car 2 nd Quarter Independent Study 1934.

***. The Air Car 2 nd Quarter Independent Study 1934.
HW2: exome sequencing and complex disease Jacquemin Jonathan de Bournonville Sébastien.

HW2: exome sequencing and complex disease Jacquemin Jonathan de Bournonville Sébastien.
LABORATORY VALIDATION OF TWO REAL TIME RT-PCR METHODS FOR FOOT-AND-MOUTH DISEASE VIRUS WITH AN INCREASED DIAGNOSTIC SPECIFICITY Vandenbussche Frank, Lefebvre.

LABORATORY VALIDATION OF TWO REAL TIME RT-PCR METHODS FOR FOOT-AND-MOUTH DISEASE VIRUS WITH AN INCREASED DIAGNOSTIC SPECIFICITY Vandenbussche Frank, Lefebvre.
Risk assessment: The Safety of Blood Products presented by Dr. Thomas R. Kreil Baxter BioScience on behalf of the PPTA Pathogen Safety Steering Committee.

Risk assessment: The Safety of Blood Products presented by Dr. Thomas R. Kreil Baxter BioScience on behalf of the PPTA Pathogen Safety Steering Committee.
Algebra 1 cc Function 4 Evaluate and write arithmetic sequences Recall: A function is relation in which each element of the domain is paired with exactly.

Algebra 1 cc Function 4 Evaluate and write arithmetic sequences Recall: A function is relation in which each element of the domain is paired with exactly.
Reliable Identification of Genomic Variants from RNA-seq Data Robert Piskol, Gokul Ramaswami, Jin Billy Li PRESENTED BY GAYATHRI RAJAN VINEELA GANGALAPUDI.

Reliable Identification of Genomic Variants from RNA-seq Data Robert Piskol, Gokul Ramaswami, Jin Billy Li PRESENTED BY GAYATHRI RAJAN VINEELA GANGALAPUDI.
Genomic analysis of three African strains of Bacillus anthracis demonstrates that they are part of the clonal expansion of an exclusively pathogenic bacterium.

Genomic analysis of three African strains of Bacillus anthracis demonstrates that they are part of the clonal expansion of an exclusively pathogenic bacterium.
。 33 投资环境 3 开阔视野 提升竞争力 。 3 嘉峪关市概况 。 3 。 3 嘉峪关是一座新兴的工业旅游城市，因关得名，因企设市，是长城文化与丝路文化交 汇点，是全国唯一一座以长城关隘命名的城市。嘉峪关关城位于祁连山、黑山之间。 1965 年建市，下辖雄关区、镜铁区、长城区， 全市总面积 2935.

。 33 投资环境 3 开阔视野 提升竞争力 。 3 嘉峪关市概况 。 3 。 3 嘉峪关是一座新兴的工业旅游城市，因关得名，因企设市，是长城文化与丝路文化交 汇点，是全国唯一一座以长城关隘命名的城市。嘉峪关关城位于祁连山、黑山之间。 1965 年建市，下辖雄关区、镜铁区、长城区， 全市总面积 2935.
ΜΕΤΑΣΥΛΛΕΚΤΙΚΗ ΦΥΣΙΟΛΟΓΙΑ ΕΡΓΑΣΤΗΡΙΟ 3. Μετασυλλεκτική Εργ3-Λιοσάτου Γ.2 ΒΙΟΛΟΓΙΚΟΙ ΠΑΡΑΓΟΝΤΕΣ ΠΟΥ ΕΠΗΡΕΑΖΟΥΝ ΤΗ ΦΘΟΡΑ ΤΩΝ ΟΠΩΡΟΚΗΠΕΥΤΙΚΩΝ Αναπνοή Η λειτουργία.

ΜΕΤΑΣΥΛΛΕΚΤΙΚΗ ΦΥΣΙΟΛΟΓΙΑ ΕΡΓΑΣΤΗΡΙΟ 3. Μετασυλλεκτική Εργ3-Λιοσάτου Γ.2 ΒΙΟΛΟΓΙΚΟΙ ΠΑΡΑΓΟΝΤΕΣ ΠΟΥ ΕΠΗΡΕΑΖΟΥΝ ΤΗ ΦΘΟΡΑ ΤΩΝ ΟΠΩΡΟΚΗΠΕΥΤΙΚΩΝ Αναπνοή Η λειτουργία.
Interpreting exomes and genomes: a beginner’s guide

Interpreting exomes and genomes: a beginner’s guide

Similar presentations

Ads by Google