1. Mind Stretcher
What parts do ALL cells have?
What kinds of cells have cell walls?
Cell Membrane, Cytoplasm, DNA (genetic material) & organelles
Plant cells, fungi & some bacteria cells

2. Spooky Microscope Trivia

3. Spooky Cells 1
My composition is white and hard, you can find me in an old graveyard.

4. QUIZ over parts & functions tomorrow!!! Start memorizing!
Microscope
Basics
QUIZ over parts & functions tomorrow!!! Start memorizing!

5. Ocular lens (Eyepiece)
Body Tube
Nosepiece
Arm
Objectives
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light
Base

6. Where you View the Specimen! Ocular lens (Eyepiece)
Focuses ocular lens
Body Tube
Rotates Objective Lenses
Nosepiece
Arm
Scanning Objective (4x)
Objectives
Low Objective (10x)
Hi Power Objective (40x)
Stage
Hold Slide
Stage Clips
Coarse Adjustment
Moves stage up/down; Use to scan for specimen
Controls light!
Diaphragm
Use in high power for Fine Adjustments
Fine Adjustment
Light
Base

7. Spooky Cells 2 Some are smooth and others are hairy,
Frankenstein's was green and scary!

8. Microscope Lenses Eyepiece Low Power (yellow) Objective Lens
Holds Ocular Lens
Magnification of 10X
Scanning (red) Objective Lens
Magnification of 4x
Used for Scanning
Use Coarse Focus Knob
Low Power (yellow) Objective Lens
Magnification of 10X
Used for Scanning
Use Coarse Focus Knob
High Power (blue) Objective Lens
Magnification of 40X
Used for Fine Focusing
Seeing the details
Use Fine Focus Knob ONLY
Do NOT use Coarse Focus Knob

9. The Light Source sends light through the slide.
The Iris Diaphragm regulates the amount of light from the light source that passes through the slide.
The coarse adjustment should only be used in scanning (4x) and low power (10x) objectives. It is used for seeing the slide.
You should use the fine adjustment while in the high power objective. It is used for detailed focus.

10. The stage clips hold the slide and are located on the stage which is moved to view different areas of the slide and specimen.
All of the objectives are held on the nosepiece which rotates to change the power of magnification.
The scanning lens is usually 4x magnification and is used for initial scanning and specimen location on the slide.

11. The low power objective lens is usually 10x magnification and is also used for scanning.
The high power lens is usually 40x or 100x and is used for detailed viewing of a specimen.

12. Spooky Cell 3 I can make some people scream and squeal,
But to Edward Cullen I am a favorite meal.

13. Brain Pop- Microscopes

14. (Write calculations & answer)
What’s my power?
To calculate the power of magnification, MULTIPLY: Ocular Lens Power x Objective Lens Power = total magnification
What is the total power of magnification for the Ocular Lens (shown) and the 40x Objective Lens (shown)?
(Write calculations & answer)

15. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?

16. SAFETY: Do’s and Don’ts of Microscope Labs!
USE TWO HANDS
A microscope should always be carried by the ARM and the BASE!

17. Do’s and Don’ts
NEVER use Coarse Focus Knob with High Power Objective Lens…it Breaks Slides!
“Ooops, I broke a slide!”
Accidents Happen. Don’t FREAK OUT!
Don’t try to clean it up by yourself!
Raise your hand and a teacher will help you!

18. Cleaning UP! Always do 5 Things! Turn off & lower stage Remove slide
Wrap cord
Replace plastic cover
Put microscope back in designated area with arm facing out

19. Spooky Cell 4 Stretch me and work me to build more bulk.
Soon you will look like the incredible hulk!

20. Let’s act it out...
1 – PRETEND to Turn on the microscope and then rotate the nosepiece to click the (low power) red-banded objective into place.
2 – Place an IMAGINARY slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer.
3 – Once you have the image in view, rotate the nosepiece to view it under different powers.
Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!
4 – When you are done, turn off the microscope and put up the slides you used.

21. Microscope Parts: Match Up!
Use the bag of cards and match up the microscope part to its function.
Quietly work with the person next to you!
Finished? Raise your hand & wait for an answer key to check it!