1. Key points: summarized from chapter 14
RNA processing

2. Outline: we will focus on mRNA processing
Overview of the Central Dogma
Introns versus exons
What is a codon
Steps of pre-mRNA processing
Eukaryotes versus prokaryotes

4. 3 steps of mRNA processing occurs in the nucleus of eukaryotes
5’ CAP
Poly-A tail
Splicing

5. The 5’Cap

8. The Poly-A Tail

9. Cleavage by endonuclease

10. Basic steps of poly-Adenylation

11. Splicing Spliceosome Requires RNA + proteins Self splicing Only RNA!
Function as enzymes
Self splicing
Only RNA!
Function as enzymes!

12. Spliceosome
Meet the spliceosome

13. Splicesome: 3 components are required
Note some important aspects of the 5’ and 3’ ends
5’ phosphodiester of G
2’ OH of A
lariat

16. Where does splicing occurs?

17. Self-Splicing mRNA’s

18. Common characteristic of self-splicing mRNA’s

19. Comments on Alternative processing methods
First: What are the methods of mRNA processing discussed so far?
5’ CAP
3’ Poly A Tail
Intron Splicing

20. Alternative processing methods
Alternative splicing
Multiple 3’cleavage sites
RNA editing

23. And alternative splicing example
Thryoid
BRAIN

25. Silencing or interfering RNAs
siRNA
miRNA

26. Onto translation chapter 15