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Key points: summarized from chapter 14
RNA processing
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Outline: we will focus on mRNA processing
Overview of the Central Dogma Introns versus exons What is a codon Steps of pre-mRNA processing Eukaryotes versus prokaryotes
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3 steps of mRNA processing occurs in the nucleus of eukaryotes
5’ CAP Poly-A tail Splicing
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The 5’Cap
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The Poly-A Tail
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Cleavage by endonuclease
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Basic steps of poly-Adenylation
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Splicing Spliceosome Requires RNA + proteins Self splicing Only RNA!
Function as enzymes Self splicing Only RNA! Function as enzymes!
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Spliceosome Meet the spliceosome
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Splicesome: 3 components are required
Note some important aspects of the 5’ and 3’ ends 5’ phosphodiester of G 2’ OH of A lariat
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Where does splicing occurs?
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Self-Splicing mRNA’s
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Common characteristic of self-splicing mRNA’s
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Comments on Alternative processing methods
First: What are the methods of mRNA processing discussed so far? 5’ CAP 3’ Poly A Tail Intron Splicing
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Alternative processing methods
Alternative splicing Multiple 3’cleavage sites RNA editing
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And alternative splicing example
Thryoid BRAIN
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Silencing or interfering RNAs
siRNA miRNA
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Onto translation chapter 15
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