1. A Fluorescent Alternative to the Synthetic Strigolactone GR24
Amanda Rasmussen, Thomas Heugebaert, Cedrick Matthys, Rik Van Deun, Francois-Didier Boyer, Sofie Goormachtig, Christian Stevens, Danny Geelen 
Molecular Plant 
Volume 6, Issue 1, Pages (January 2013)
DOI: /mp/sss110
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Generalized Structure of Natural SLs and Examples of Synthetic Analogs, including the Fluorescent CISA-1.
Molecular Plant 2013 6, DOI: ( /mp/sss110)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

3. Figure 2 Synthetic Route to Precursor 10.
Molecular Plant 2013 6, DOI: ( /mp/sss110)
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4. Figure 3 Synthesis of CISA-1.
Molecular Plant 2013 6, DOI: ( /mp/sss110)
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5. Figure 4
Chemical Stability of GR24.GR24 (A) and CISA1 (B) in Methanol:Water 1:3 (v/v) at pH 5.9, 7.4 and 8.5.
Molecular Plant 2013 6, DOI: ( /mp/sss110)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

6. Figure 5
Excitation (A, C, E) and Emission (B, D, F) Curves for Compound 9 (A, B), Compound 10 (C, D), and CISA-1 (E, F). Chemical structures for CISA-1 are given in Figure 1 and compounds 9 and 10 are given in Figure 2.
Molecular Plant 2013 6, DOI: ( /mp/sss110)
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7. Figure 6
Increasing Levels of CISA-1 Increase Orobanche Germination in a Dose-Dependent Manner. Means are presented±standard error. Student’s t-tests were used to compare germination at each concentration (** P<0.01).
Molecular Plant 2013 6, DOI: ( /mp/sss110)
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8. Figure 7
CISA-1 Inhibits Adventitious Roots More Efficiently than GR24 and in a MAX2-Dependent Manner. Wild-type (col) (A) and max4 (B) strigolactone-deficient mutant seedlings responded more to the strigolactone analog CISA-1 compared to GR24. Student’s t-tests were used to compare the two compounds at each concentration at the p<0.05 (*), 0.01 (**), and (***) levels.(C) max1, max4, strigolactone-deficient mutants; max2, strigolactone-insensitive mutant. Different letters represent significantly different means at P<0.05 (Student’s t-tests). In all panels, n>30 and means are presented±standard error bars.
Molecular Plant 2013 6, DOI: ( /mp/sss110)
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9. Figure 8
CISA-1 Reduces the Number of Inflorescence Stems.col, Columbia wild-type; max4, strigolactone-deficient mutant; max2, strigolactone-insensitive mutant. Different letters represent significantly different means at P<0.05 (Student’s t-tests; n>8). Means are presented±standard error bars.
Molecular Plant 2013 6, DOI: ( /mp/sss110)
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10. Figure 9
MAX4 Expression in Arabidopsis Roots Is Suppressed by CISA-1. Means of four replicates are presented±standard deviation bars. Each replicate contained roots from 50 seedlings. Means of both GR24 and CISA-1-treated seedlings were significantly different to the control seedlings at 24h and 48h after treatment. (*** significant at P<0.001).
Molecular Plant 2013 6, DOI: ( /mp/sss110)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

11. Figure 10 Molecular Overlap of GR24 (Yellow) and CISA-1 (Blue).
Molecular Plant 2013 6, DOI: ( /mp/sss110)
Copyright © 2013 The Authors. All rights reserved. Terms and Conditions