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Proteomic analysis of sputum in patients with active pulmonary tuberculosis
Y.R. Fu, Z.J. Yi, S.Z. Guan, S.Y. Zhang, M. Li Clinical Microbiology and Infection Volume 18, Issue 12, Pages (December 2012) DOI: /j x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions
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FIG. 1 Protein patterns of sputum. (a) A representative TB example is shown. (b) A representative control sample is shown. The resulting two samples containing 150 μg protein were added to pH 3–10, non-linear DryStrips (24 cm long; Bio-Rad, Hercules, CA, USA). The p values for these spots in three independent experiments were <0.05. Clinical Microbiology and Infection , DOI: ( /j x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions
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FIG. 2 The result of the matrix-assisted laser desorption/ionization-time of flight/time of flight-mass spectrometry MALDI-TOF/TOF-MS analysis of the protein IL-25. It was identified to be human IL-25 (NCBI accession number: ) by protein database search. (a) Peptide mass fingerprint. (b) MS/MS profile of the peptide with a mass of Da; amino acids indicated as ASEDGPLNSR; confidence score %. Clinical Microbiology and Infection , DOI: ( /j x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions
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FIG. 3 Confirmation of IL-25 expression by ELISA. The data showed that IL-25 had an increased abundance in the TB group compared with the control group. #The p value for IL-25 was <0.05 in the active TB group compared with the controls. Clinical Microbiology and Infection , DOI: ( /j x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions
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