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Expression of cytoskeleton and energetic metabolism-related proteins at human abdominal aortic aneurysm sites  Javier Modrego, PhD, Antonio J. López-Farré,

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Presentation on theme: "Expression of cytoskeleton and energetic metabolism-related proteins at human abdominal aortic aneurysm sites  Javier Modrego, PhD, Antonio J. López-Farré,"— Presentation transcript:

1 Expression of cytoskeleton and energetic metabolism-related proteins at human abdominal aortic aneurysm sites  Javier Modrego, PhD, Antonio J. López-Farré, PhD, Isaac Martínez-López, MD, Miguel Muela, MD, Carlos Macaya, MD, PhD, Javier Serrano, MD, PhD, Guillermo Moñux, MD, PhD  Journal of Vascular Surgery  Volume 55, Issue 4, Pages (April 2012) DOI: /j.jvs Copyright © 2012 Society for Vascular Surgery Terms and Conditions

2 Fig 1 Mass spectrometry (MS) and tandem mass spectrometry spectra (MS/MS) to identify proteins. Spectra of triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, and annexin A2. The matched peptides are shown in the figure for each specific protein and they were analyzed using the Mascot database ( for the identity of each protein. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Representative bidimensional gel electrophoresis (2-DE) of an abdominal aortic proteome. Proteomes were developed using a pH range between 3 and 10. In the gel is marked the spots that were analyzed. On the right is shown representative spots of cytoskeleton and energetic metabolism-related proteins in which a different level of expression was found between the abdominal aortic aneurysm (AAA) site and control abdominal aortic samples. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

4 Fig 3 Representative Western blots of triosephosphate isomerase and pyruvate dehydrogenase. Bar graphs show the densitometric analysis in arbitrary units (AU) of the Western blots. The densitometric analysis was performed using linear regression using age as covariant. The protein expression as response variable and the aortic disease status as independent variable. Densitometric values are represented as mean ± SEM. *P < .05 with respect to control. AAA, Abdominal aortic aneurysm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

5 Fig 4 Enzymatic activities and metabolites measures of key-step glycolytic enzymes. On the top is shown graphs of 1,6 biphosphate aldolase and triosephosphate isomerase activities in control abdominal aortic sample and at the abdominal aortic aneurysm (AAA) site. At the bottom is shown pyruvate and lactate content at the AAA site and in control abdominal aortic samples. Enzymatic activity and pyruvate and lactate content were considered as response variable, aortic disease status as independent variable and age was used as covariant. Results are represented as mean ± SEM. *P < .05. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Enzymatic activity of lactate dehydrogenase. The enzymatic activity was determined in time 0 and 30 minutes after add the homogenate tissue to reactive reagents as manufacturer's recommended. Lactate dehydrogenase activity was used as response variable, aortic state as independent value, and age as covariant in the linear regression model. Results are mean ± SEM. AAA, Abdominal aortic aneurysm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2012 Society for Vascular Surgery Terms and Conditions

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