1. nramp2 Mutants Are Hypersensitive to Mn Deficiency.
nramp2 Mutants Are Hypersensitive to Mn Deficiency. (A) Position of the T-DNA insertion in two nramp2 mutant alleles relative to the ATG start codon. (B) Relative NRAMP2 transcript abundance measured by qRT-PCR in nramp2 mutant alleles and in the two nramp2-3 complemented lines OxNR2 #3 and OxNR2 #6. Transcript levels are expressed relative to those of the reference gene ACTIN ±sd (n = 3 measurements per sample). (C) Phenotypes of plants of the indicated genetic backgrounds grown for one week in Mn-replete conditions (5 µM Mn SO4) followed by three additional weeks in Mn-free hydroponic conditions. Bars = 1 cm. (D) and (E) Shoot (D) and root (E) biomass production of the plants presented in (C). Data are from one representative experiment (n = 10 plants). (D) and (E) share color code legends. Mean ± sd. Asterisks indicate values significantly different from those of the wild type (Student’s t test, *P value < 0.05 and **P value < 0.01). DW, dry weight.
Santiago Alejandro et al. Plant Cell 2017;29:
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