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Correlation between DNA defect and sperm-head morphology

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Presentation on theme: "Correlation between DNA defect and sperm-head morphology"— Presentation transcript:

1 Correlation between DNA defect and sperm-head morphology
Nino G Cassuto, André Hazout, Ibrahim Hammoud, Richard Balet, Dominique Bouret, Yona Barak, Sonia Jellad, Jean Marie Plouchart, Jacqueline Selva, Chadi Yazbeck  Reproductive BioMedicine Online  Volume 24, Issue 2, Pages (February 2012) DOI: /j.rbmo Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

2 Figure 1 High-magnification-score-0 spermatozoon, showing an abnormal head with a vacuole and an abnormal base. Bar=5μm. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

3 Figure 2 Spermatozoa scored at high magnification according to the Cassuto–Barak classification. Bar=5μm. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

4 Figure 3 Sperm-scoring sketches according to the Cassuto–Barak classification: 2HN+3VN+1BN (BN=normal base; HN=normal head; VN=no vacuole(s)), maximum score is 6. (a) Score 6: HN+VN+BN; (b) score 5: HN+VN; (c) score 4: VN+BN; (d and e) score 3: HN+BN (f) score 3: VN; (g) score 2: HN; (h) score 1: BN; (i) score 0. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

5 Figure 4 Score-6 spermatozoon, showing normal head without a vacuole with a normal base. Bar=5μm. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

6 Figure 5 Study protocol for 26 men. From each semen sample, 100 score-0 spermatozoa were placed on each of two slides next to drops of 100 total migrated spermatozoa (TMS) (control). A total of 400 spermatozoa were examined per patient and 10,400 spermatozoa for DNA fragmentation (DF) and sperm chromatin decondensation (SCD). HM=high magnification; S=score; Spz=spermatozoa. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

7 Figure 6 Regression of sperm chromatin decondensation (SCD) with score-0 spermatozoa in total migrated spermatozoa (n=26). Regression equation: %SCD= ×(%score 0). 90% confidence intervals are shown. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

8 Figure 7 Score-0 spermatozoon at high and low magnification. Bar=5μm.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

9 Figure 8 Score-5 spermatozoon with an abnormal base at high and low magnification. Bar=5μm. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

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