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C. Keith Ozaki, MD, Matthew D. Phaneuf, BS, Suchen L

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Presentation on theme: "C. Keith Ozaki, MD, Matthew D. Phaneuf, BS, Suchen L"— Presentation transcript:

1 Glycoconjugate mediated endothelial cell adhesion to Dacron polyester film* 
C.Keith Ozaki, MD, Matthew D. Phaneuf, BS, Suchen L. Hong, PhD, William C. Quist, MD, PhD, Frank W. LoGerfo, MD  Journal of Vascular Surgery  Volume 18, Issue 3, Pages (September 1993) DOI: / (93)90267-P Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 EDC-mediated conjugation of UEA I to PET.
Journal of Vascular Surgery  , DOI: ( / (93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Schematic demonstrates biotinylated neoglycoprotein fucosylamide albumin binding to immobilized lectin. This interaction is documented with fluorescent avidin. Journal of Vascular Surgery  , DOI: ( / (93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Binding of Na[125I]-labeled UEA I to hydrolyzed PET with cross-linker EDC. Covalent = (Covalent + Nonspecific) − nonspecific. Journal of Vascular Surgery  , DOI: ( / (93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 IAEC adherence to various PET surfaces (n = 4 independent experiments for each). Significantly more cells bound to the UEA I/PET surface than to any control surface (Tukey-Kramer multiple comparisons). Journal of Vascular Surgery  , DOI: ( / (93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 Each of five vascular endothelial cell lines tested adhered to UEA I/PET surface, whereas monocytes, SMCs, and fibroblasts did not (n = 4 independent experiments for each). Journal of Vascular Surgery  , DOI: ( / (93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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