1. Photosynthesis By: Chloe & Christina

2. *remember the 1st law of thermodynamics
Photosynthesis is the process by which AUTOTROPHS (plants) convert the energy in SUNLIGHT into the energy stored in ORGANIC COMPOUNDS.
*remember the 1st law of thermodynamics
The 1st Law of Thermodynamics states that “energy cannot be created or destroyed, but it can be transformed from one form to another.”

3. Photosynthesis & Ecology
10% Rule
The energy captured through photosynthesis forms the basis of the ecological pyramid.
The biomass that producers make supports almost all life on Earth.
Autotrophs capture energy & fix it into organic compounds, heterotrophs consume these organic compounds.

4. Leaves: The Photosynthetic Organs
Leaves perform most of the photosynthesis in plants
They have a large surface area to absorb as much sunlight as possible
Cuticle- A thin, waxy layer that covers the upper epidermis of the leaf, preventing the loss of water.
Palisade Layer- Cells that contain chloroplasts and absorb a major portion of the light energy used in photosynthesis.
Spongy Layer- Cells containing chloroplasts, major site of photosynthesis.
Air Spaces- Spaces between spongy mesophyll cells for air and gas circulation (CO2 & O2).
Stomata- An opening in the lower epidermis that allows carbon dioxide into the leaf and water and oxygen out of the leaf.
Vein- Consists of the Xylem and Phloem. Xylem cells carry water and minerals up from the roots through the stem and into the leaf, and Phloem cells carry sugar and starch from the leaf down through the stem and into the roots for storage.

5. Chloroplast Structure
Chloroplasts are the site of photosynthesis in eukaryotes and are located in the leaves of plants
3 membranes fold to form thylakoid, which are arranged in stacks called grana
Chlorophyll and other pigments are contained in the thylakoid membrane
3 Membranes
Outer Membrane
Inner Membrane
Thylakoid Membrane

7. Light & Pigments
Visible light is made up of different colors of light containing different wavelengths
Pigments are molecules that absorb light energy
Chlorophyll A
The dominant pigment in photosynthesis and absorbs best in the red and blue wavelengths, least the green
Chlorophyll B
Accessory pigment that captures additional light energy which is then transferred to chlorophyll a. It occurs in most plants but not all.
Carotenoids
Includes Xanthophyll and Carotenes.
Accessory pigments in photosynthesis.

9. Photosynthesis Equation (Overview)
Net overall equation:
6CO2+6H2O C6H12O6+6O2
Light transfer in photosynthesis:
light
Light ATP & NADPH Organic Compounds
Light reactions
thylakoids
Calvin Cycle
stroma

10. Phase 1: Light Dependent Reactions

11. 2 photosystems are involved:
Light reactions of photosynthesis involve the use photosystems*
*a cluster of pigment molecules bound to proteins, along with a primary electron acceptor
2 photosystems are involved:
Photosystem II (P680)- Absorbs lights best at a wavelength of 689nm
Photosystem I (P700)- Absorbs lights best at a wavelength of 700nm

12. Non-Cyclic Electron Flow

13. Non-Cyclic Electron Flow
Step1: A photon of light excites a chlorophyll molecule in Photosystem II and is transferred to other pigment molecules until it reaches the reaction site and the primary electron acceptor captures the electrons, exciting the electron to a higher energy level.

14. Non-Cyclic Electron Flow
Step 2: The P680 molecule is missing two electrons, so water is split and gives electrons to P680 while O2 is given off as a byproduct.

15. Non-Cyclic Electron Flow
Step 3: The excited electron then travels down the electron transport chain, losing energy as it goes. This energy is used to build a concentration gradient of protons for chemiosmosis*. At this point, Photosystem I also absorbs light energy, exciting one of its electrons to a higher energy level.
*the process that makes ATP (Adenine, 5 carbon sugar, 3 phosphate groups)

16. Non-Cyclic Electron Flow
Step 4: The electron lost in the beginning of Photosystem II replaces the lost electron in Photosystem I. This excited electron then travels down the electron transport chain, once again losing energy, and eventually reduced NADP+ to NADPH (electron carrier).

17. Chemiosmosis
Protons (H+) are pumped from the stroma in the chloroplast across a semipermeable membrane into thylakoid space. In Chemiosmosis, the proton diffuse back across the membrane through the enzyme ATP Synthase, causing ATP Synthase to spin and force ADP to combine with another phosphate group, forming ATP. This ATP is used as energy for the light independent reactions, or the Calvin Cycle.

18. Cyclic Electron Flow Used in more primitive organisms.
Only photosystem I is used.
Instead of splitting water to replace the lost electrons, the electron is recycled back into the photosystem.
Oxygen is not released and NADPH is not produced.
Because the Calvin Cycle requires more ATP than NADPH, the cyclic electron flow compensates for not making NADPH.

19. Light Independent Reactions

20. Overview
In the Calvin Cycle, chemical energy (from the light reactions) and CO2 (from the atmosphere) are used to produce organic compounds (like glucose).
It happens in the stroma of the chloroplasts.

21. Before we begin...
Know that in this slideshow (and many diagrams) discuss the Calvin Cycle in terms of 1 G3P made, not of 1 glucose made.
Also the Barron’s book mentions that the exam likely won’t have all the fine details of the Calvin Cycle like on the right diagram

22. Step 1: Carbon Fixation
3 molecules of CO2 are brought in from the atmosphere. Each is combined with a 5-carbon sugar called RuBP. This forms 3 very unstable 6-molecules.

23. Step 2: Reduction
The 6-carbon molecules each break into two 3-carbon molecules, called 3-PGA.
Then, six NADPH and six ATP are used to reduce all the six 3- PGA into six G3P molecules.

24. Step 3: Regeneration
There are now six 3-carbon molecules. Since the Calvin Cycle started with 15 carbons (three 5-carbon molecules) and there are now 18 carbons, we have a net gain of 3 carbons.
One G3P molecule is “gained” in this process. It can be used to produce organic compounds (like half of a glucose molecule). The other 5 must be reformed to continue the cycle.

25. Step 3: Regeneration
Five of the G3P are recycled back into three RuBP. This requires three ADP molecules.
Notice that three RuBP molecules were required to start the cycle in the first step!

27. Photorespiration

28. Makes compounds that must be broken apart
What if there’s no CO2?
If there is no CO2 for rubisco to bind with, it will instead bind to O2. This is called photorespiration.
Makes compounds that must be broken apart
Doesn’t make any sugar
Uses up ATP

29. Photorespiration Occurrence
Photorespiration is primarily a problem when plants are dry. When plants are under water stress, their stomata close to prevent water loss through transpiration. However, this also limits gas exchange.
Some plants have special adaptations against this.
Photorespiration Occurrence

30. C-4 Photosynthesis
In C4 plants, the light-dependent reactions and the Calvin cycle are physically separated, with the light- dependent reactions occurring in the mesophyll cells (spongy tissue in the middle of the leaf) and the Calvin cycle occurring in bundle-sheath cells.

31. At night, CAM plants open their stomata, allowing CO2 to diffuse into the leaves.
This CO2 is fixed into an organic acid and stored until the next day.
In the daylight, the CAM plants do not open their stomata. That's because the organic acids are broken down to release CO2, which enters the Calvin cycle. This controlled release maintains a high concentration CO2 around rubisco.
CAM Plants

32. Q U I Z
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