1. Supplementary Figure 1 Non-migrating cells Migrating cells
Ovarian cancer cell line
(1) Transwell migration assay
(3) Orthotopic implantation
(4) Selection of non-metastatic
and metastatic clones
(2) Clonal propagation
# # #3 …
Clone

2. Supplementary Figure 2 a b c d e NM HM NM HM NM HM NM HM
Comparison of tumorigenic and metastatic abilities
Cells Tumor No. of mice Malignant
formation with metastasis ascites (ml)
NM Yes (4/4) /
HM Yes (4/4) / ± 0.5
50μm d NM HM
Cell number (105)
50μm
Days in culture e
SKOV3.ip1 NM HM

3. Supplementary Figure 3 a b c NM HM Number of passages
Number of spheroids c
Oct4
GAPDH
Bmi-1
ABCG2
SKOV3.ip1 NM HM

4. Supplementary Figure 4 a b NES = 1.53, FDR = 0.002

5. Supplementary Figure 5 NM
Nanog
DAPI
β-catenin
Merge HM
50μm

6. Supplementary Figure 6 a b β-catenin siRNA NS siRNA % migrated cells *
Dicer
β-catenin
siRNA
NS siRNA
β-actin
β-catenin
siRNA
NS siRNA
kDa
200
140
Dicer
100
β-catenin 80
GAPDH
100 80 50 40

7. Supplementary Figure 7 a b c d HEYA8 NM NM #10 NM #11 HM HM #6 HM #8
kDa
Dicer
200
140
100 80
200
Drosha
140
100 80 50
β-actin 40 b
miR-29a
miR-29c U6
miR-29b
HEYA8 NM
NM #10
NM #11 HM
HM #6
HM #8 c d
miR-29a
miR-29b
miR-29c NM HM NS
E-cadherin
E-cadherin
Snail
Snail
N-cadherin
N-cadherin
GAPDH
GAPDH

8. Supplementary Figure 8 Marker NS siRNA β-catenin siRNA Marker NS siRNA
Dicer siRNA
kDa
kDa
200
200
Dicer (220kDa)
(short exposure)
140
140
100
100 80
β-catenin (92kDa)
(short exposure) 80 60 60 *
200
200
Dicer
(long exposure)
140
140
100
100
β-catenin
(long exposure) 80 80 60 60 50 * 50 40 40 60 60 50 50
β-actin (42kDa)
β-actin
(42 kDa) 40 40 30 30