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PublishHomer Job Barnett Modified over 5 years ago
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Supplementary Figure 1 Non-migrating cells Migrating cells
Ovarian cancer cell line (1) Transwell migration assay (3) Orthotopic implantation (4) Selection of non-metastatic and metastatic clones (2) Clonal propagation # # #3 … Clone
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Supplementary Figure 2 a b c d e NM HM NM HM NM HM NM HM
Comparison of tumorigenic and metastatic abilities Cells Tumor No. of mice Malignant formation with metastasis ascites (ml) NM Yes (4/4) / HM Yes (4/4) / ± 0.5 50μm d NM HM Cell number (105) 50μm Days in culture e SKOV3.ip1 NM HM
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Supplementary Figure 3 a b c NM HM Number of passages
Number of spheroids c Oct4 GAPDH Bmi-1 ABCG2 SKOV3.ip1 NM HM
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Supplementary Figure 4 a b NES = 1.53, FDR = 0.002
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Supplementary Figure 5 NM Nanog DAPI β-catenin Merge HM 50μm
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Supplementary Figure 6 a b β-catenin siRNA NS siRNA % migrated cells *
Dicer β-catenin siRNA NS siRNA β-actin β-catenin siRNA NS siRNA kDa 200 140 Dicer 100 β-catenin 80 GAPDH 100 80 50 40
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Supplementary Figure 7 a b c d HEYA8 NM NM #10 NM #11 HM HM #6 HM #8
kDa Dicer 200 140 100 80 200 Drosha 140 100 80 50 β-actin 40 b miR-29a miR-29c U6 miR-29b HEYA8 NM NM #10 NM #11 HM HM #6 HM #8 c d miR-29a miR-29b miR-29c NM HM NS E-cadherin E-cadherin Snail Snail N-cadherin N-cadherin GAPDH GAPDH
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Supplementary Figure 8 Marker NS siRNA β-catenin siRNA Marker NS siRNA
Dicer siRNA kDa kDa 200 200 Dicer (220kDa) (short exposure) 140 140 100 100 80 β-catenin (92kDa) (short exposure) 80 60 60 * 200 200 Dicer (long exposure) 140 140 100 100 β-catenin (long exposure) 80 80 60 60 50 * 50 40 40 60 60 50 50 β-actin (42kDa) β-actin (42 kDa) 40 40 30 30
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