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Chromatin Starts to Come Clean

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1 Chromatin Starts to Come Clean
Shengjiang Tu, Gary LeRoy, Danny Reinberg  Molecular Cell  Volume 64, Issue 3, Pages (November 2016) DOI: /j.molcel Copyright © 2016 Elsevier Inc. Terms and Conditions

2 Figure 1 Schematic Diagram of ChIP-SICAP
(A) Formaldehyde cross-linked sheared chromatin prepared from cells. (B) Immunoprecipitation of nucleosomal chains and associated proteins with antibody specific to target protein. (C) Wash immunoprecipitate followed by addition of Biotin-ddUTP by TdT. (D) Precipitate with Strepavidin beads. (E) Successive stringent washes followed by elution and reversal of cross-links. (F) Proteins are trypsinized and analyzed by quantitative mass spectrometry. Molecular Cell  , DOI: ( /j.molcel ) Copyright © 2016 Elsevier Inc. Terms and Conditions

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