1. Hepatitis C Virus Replicons Volume 3 and 4
Volker Lohmann, Ralf Bartenschlager 
Gastroenterology 
Volume 144, Issue 1, Pages (January 2013)
DOI: /j.gastro
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2. Figure 1
Method used to establish subgenomic replicon-harboring Huh7 cells. Replicons consist of the HCV 5′ nontranslated region (NTR), the selection marker neomycin phosphotransferase (neo), conferring resistance to the cytostatic drug G418, the internal ribosome entry site of the encephalomycarditis virus (EMCV-IRES) driving translation of the NS3 to NS5B coding sequence of HCV and the 3′ NTR. Synthetic replicon RNA molecules are transfected into the human hepatoma cell line Huh7. Upon selection with G418, only those cells that support efficient RNA replication will survive and form a stable replicon cell clone. Efficient RNA replication of genotype 1, 3, and 4 replicons depends on the acquisition of replication enhancing (adaptive) mutations, which is indicated by asterisks and by a color change of the coding sequence. Cells that are either not transfected or that do not support efficient RNA replication are eliminated by G418 treatment.
Gastroenterology  , 13-15DOI: ( /j.gastro )
Copyright © 2013 AGA Institute Terms and Conditions