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Published byΠλούταρχος Λιάπης Modified over 6 years ago
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First-instar thrips larvae Second-instar thrips larvae
Size thrips larvae Size of thrips larvae The size of thrips larvae was measured using a binocular microscope with an ocular graticule. We measured length, width and height of ten small first-instar larvae (freshly hatched from eggs), ten large first-instar larvae (about to molt into second instar larvae), ten small second-instar larvae (just molted from first instar) and ten large second-instar larvae (about to molt into prepupae) Thrips larvae were measured on a cucumber leaf in a Petri dish (as described above for the thrips culture); length and width were measured with the Petri dish in a horizontal position, height was measured with the Petri dish in a vertical position. First-instar thrips larvae Second-instar thrips larvae Freshly hatched Before molting After molting Before pupation Length 0.50 ± mm 0.92 ± mm 0.92 ±0.03 mm 1.16 ±0.02 mm Width 0.11 ± mm 0.20 ± mm 0.21 ± 0.01 mm 0.25 ± 0.01 mm Height 0.10 ± mm 0.13 ± mm 0.15 ± 0.01 mm 0.22 ± 0.01 mm Table S1. Length, width and height of thrips larvae in mm (mean ± se) .
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Table s2 N=150 for all categories First-instar larvae
Second-instar larvae before treatment after treatment Wilcoxon signed rank test before treatment after treatment Wilcoxon signed rank test Behavior Excretion of anal droplet 0.45 ± 0.04 0.48 ± 0.04 V = 1039, P = 0.63 0.24 ± 0.04 0.27 ± 0.04 V = 539, P = 0.57 Swinging abdominal 1.06 ± 0.12 0.79 ± 0.10 V = 2658, P = 0.06 1.51 ± 0.19 0.90 ± 0.12 V = 2952, P < 0.001 Partially crossing the edge of the arena 0.55 ± 0.08 1.94 ± 0.16 V = 521, P < 0.001 0.73 ± 0.09 2.22 ± 0.21 V = 1081, P < 0.001
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Table s3 First-instar larvae Second-instar larvae Behavior
before treatment after treatment Wilcoxon signed rank test before treatment after treatment Wilcoxon signed rank test n n Behavior Excretion of anal droplet 123 0.34 ± 0.04 0.51 ± 0.12 V = 656, P = 0.95 123 0.2 ± 0.04 0.43 ± 0.11 V = 441, P = 0.16 Swinging abdominal 126 0.30 ± 0.06 0.71 ± 0.01 V = 424, P < 0.001 121 0.48 ± 0.07 0.97 ± 0.15 V = 559, P < 0.01 Partially crossing the edge of the arena 123 0.32 ± 0.09 4.94 ± 0.29 V = 102.5, P < 0.001 125 0.42 ± 0.08 8.40 ± 0.74 V = 47.5, P < 0.001
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