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Perigraft vascularization and incorporation of implanted Dacron prostheses are affected by rifampicin coating  Mohammed R. Moussavian, MD, Matthias W.

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Presentation on theme: "Perigraft vascularization and incorporation of implanted Dacron prostheses are affected by rifampicin coating  Mohammed R. Moussavian, MD, Matthias W."— Presentation transcript:

1 Perigraft vascularization and incorporation of implanted Dacron prostheses are affected by rifampicin coating  Mohammed R. Moussavian, MD, Matthias W. Laschke, MD, Georg Schlachtenberger, Maximilian von Heesen, MD, Matthias Wagner, MD, Matthias Glanemann, MD, Michael D. Menger, MD  Journal of Vascular Surgery  Volume 64, Issue 6, Pages (December 2016) DOI: /j.jvs Copyright © 2015 Society for Vascular Surgery Terms and Conditions

2 Fig 1 Intravital fluorescent microscopic images of the border of (A) a Dacron-Con (asterisk) and (B) a Dacron-Rifamp graft (asterisk) at day (d) 14 after implantation into the dorsal skinfold chamber of C57BL/6 mice. Note the high density of newly formed microvessels at the border of (A) Dacron-Con (arrows). B, Dacron-Rifamp shows only a few newly formed microvessels (arrows). Blue light epi-illumination with contrast enhancement by 5% fluorescein isothiocyanate-labeled intravenous dextran 150,000. C, Functional capillary density (cm/cm2) within the border of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n = 8), assessed using intravital fluorescence microscopy and computer-assisted image analysis. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. A and B, Scale bar = 500 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Immunohistochemical cross sections of the border zones of (A) a Dacron-Con and (B) a Dacron-Rifamp graft at day 14 after implantation in the striated muscle tissue within the dorsal skinfold chamber of C57BL/6 mice. The sections were stained with Hoechst 33,342 to identify cell nuclei (blue) and with an antibody against CD31 for the visualization of microvessels (red). Note the higher microvessel density in the border zone of the (A) Dacron-Con compared with the (B) Dacron-Rifamp graft. C, Microvessel density (mm2) in the border zones of Dacron-Con (white bar; n = 8) and Dacron-Rifamp grafts (black bar; n = 8) at day 14 after implantation. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. A and B, Scale bar = 125 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

4 Fig 3 A, Rolling leukocytes (per minute) and (B) adherent leukocytes (mm2) in postcapillary and collecting venules within the border zones of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n = 8), assessed using intravital fluorescence microscopy and computer-assisted image analysis. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. d, Day. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

5 Fig 4 Immunohistochemical cross sections of the border zones of (A-C) a Dacron-Con and (D-F) a Dacron-Rifamp graft at day 14 after implantation into the striated muscle tissue within the dorsal skinfold chamber of C57BL/6 mice. The sections were stained with an antibody against myeloperoxidase (MPO) for the detection of neutrophils (A and D, arrows), an antibody against CD3 for the detection of lymphocytes (B and E, arrow), and an antibody against MAC387 for the detection of macrophages (C and F, arrows). G-I, MPO-positive neutrophils (mm2), CD3-positive lymphocytes (mm2), and MAC387-positive macrophages (mm2) in the border zones of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n =8) at day 14 after implantation. Mean ± standard error of the mean. A-F, Scale bar = 125 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Immunohistochemical cross sections of the border zones of (A) a Dacron-Con and (B) a Dacron-Rifamp graft at day 14 after implantation into the striated muscle tissue within the dorsal skinfold chamber of C57BL/6 mice. The sections were stained with an antibody against Ki-67 for the visualization of proliferating cells (arrows). C, Ki-67-positive cells (%) in the border zones of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n = 8) at day 14 after implantation. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. A and B, Scale bar = 125 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

7 Fig 6 Immunohistochemical cross sections of the border zones of (A) a Dacron-Con and (B) a Dacron-Rifamp graft at day 14 after implantation into the striated muscle tissue within the dorsal skinfold chamber of C57BL/6 mice. The sections were stained with an antibody against caspase-3 (casp-3) for the visualization of apoptotic cells (arrows). C, casp-3-positive cells (%) in the border zones of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n = 8) at day 14 after implantation. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. A and B, Scale bar = 125 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

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