Presentation is loading. Please wait.

Presentation is loading. Please wait.

Investigation of peanut oral immunotherapy with CpG/peanut nanoparticles in a murine model of peanut allergy  Kamal D. Srivastava, PhD, Alyssa Siefert,

Published byFrieder Sommer Modified over 6 years ago

Similar presentations

Presentation on theme: "Investigation of peanut oral immunotherapy with CpG/peanut nanoparticles in a murine model of peanut allergy  Kamal D. Srivastava, PhD, Alyssa Siefert,"— Presentation transcript:

1 Investigation of peanut oral immunotherapy with CpG/peanut nanoparticles in a murine model of peanut allergy  Kamal D. Srivastava, PhD, Alyssa Siefert, PhD, Tarek M. Fahmy, PhD, Michael J. Caplan, MD, Xiu-Min Li, MD, Hugh A. Sampson, MD  Journal of Allergy and Clinical Immunology  Volume 138, Issue 2, Pages e4 (August 2016) DOI: /j.jaci Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Experimental design. Protocol for sensitization, treatment, and challenge: 6-week-old C3H/HEJ mice were orally sensitized with peanut (PN) and cholera toxin at weeks 0 through 5. Mice were boosted with peanut and cholera toxin at weeks 6 and 8. Weekly oral treatment started at week 11 (blue upward arrows) and continued through week 14. Mice underwent oral peanut challenge 5 days later and then at 4-week intervals for another 4 challenges (weeks 14, 18, 22, 26, and 30, as indicated by tall black arrows). Short black arrows indicate oral gavage with sensitization dose of peanut plus cholera toxin given 1 day after each challenge, except the final challenge at week 30. N = 10-22 mice per group from 2 separate experiments. i.g., Intragastric; NP, nanoparticles. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Anaphylaxis symptom scores. Thirty minutes after oral peanut (PN) challenge, mice were visually assessed for symptoms of anaphylaxis and assigned symptom scores using the scoring system described in the Methods section. Naive mice were challenged only at the fifth challenge. Bars represent group medians. **P < .01 and ***P < .001 versus sham. N = 10-22 mice per group from 2 separate experiments. NC, Not challenged; NP, nanoparticles. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Body temperature after oral peanut (PN) challenges. After recording symptom scores, the core body temperature of each animal was measured with a rectal thermometer. Data show group medians and interquartile range. *P < .05 and ***P < .001 versus sham. N = 10-22 mice per group from 2 separate experiments. NP, Nanoparticles. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Plasma histamine levels after oral peanut (PN) challenge. Blood was drawn approximately 30 minutes after the oral peanut challenge, and plasma was harvested for measurement of histamine by using ELISA. Data are shown as means ± SDs at each of the 5 challenges. **P < .01 and ***P < .001 versus sham. N = 10-22 mice per group from 2 separate experiments. NP, Nanoparticles. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Peanut (PN)–specific immunoglobulins. Blood was drawn from mice at the times indicated. Peanut-specific serum IgE (A), IgG1 (B), and IgG2a (C) levels were measured by using ELISA. Data symbols indicate group medians, and bars indicate interquartile ranges. *P < .05 and **P < .01 versus sham. N = 10-22 mice per group from 2 separate experiments. NP, Nanoparticles. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 Peanut (PN)–restimulated splenocyte cytokine levels. Splenocyte cultures from individual mice were incubated in the presence of 200 μg of peanut extract or medium alone for 72 hours under standard tissue culture conditions. Cytokine levels were measured by using ELISA. Data are displayed as group medians and interquartile ranges. ***P < .001 versus sham. N = 10-22 mice per group from 2 separate experiments. NP, Nanoparticles. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E1 Anaphylactic reactions to oral peanut (PN) challenge. A, Mice were visually assessed for symptoms of anaphylaxis 30 minutes after challenge and assigned symptom scores by using the scoring system described in the Methods section. Naive mice were challenged only at the fifth challenge. Bars represent group medians. B, Body temperatures were measured with a rectal thermometer. C, Histamine levels in plasma harvested after challenge were measured by using ELISA. Data are shown as means ± SDs. Bars indicate group medians in Fig E1, A, and group means ± SDs for Fig E1, B and C. *P < .05, **P < .01, and ***P < .001 versus sham. N = 5-10 mice per group. NP, Nanoparticles. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Fig E2 Peanut (PN)–specific immunoglobulins. Blood was drawn before treatment (week 11) and each of the challenges (weeks 14-30). Peanut-specific serum IgE (A), IgG1 (B), and IgG2a (C) levels were measured by using ELISA. Symbols indicate group medians, and bars indicate interquartile ranges. **P < .01; ***P < .001 versus sham. N = 5-10 mice per group. NP, Nanoparticles. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 Fig E3 Peanut (PN)–restimulated splenocyte cytokine levels. Splenocyte cultures from individual mice were incubated in the presence of 200 μg of peanut extract or medium alone for 72 hours under standard tissue culture conditions. Cytokine levels were measured by using ELISA. Data show group medians and interquartile ranges. ***P < .001 versus sham. N = 5-10 mice per group. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Investigation of peanut oral immunotherapy with CpG/peanut nanoparticles in a murine model of peanut allergy  Kamal D. Srivastava, PhD, Alyssa Siefert,"

Similar presentations

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS 

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS 
Dose-time-response relationship in peanut allergy using a human model of passive cutaneous anaphylaxis  Anja P. Mose, MD, Charlotte G. Mortz, MD, PhD,

Dose-time-response relationship in peanut allergy using a human model of passive cutaneous anaphylaxis  Anja P. Mose, MD, Charlotte G. Mortz, MD, PhD,
Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.

Repeated low-dose intradermal allergen injection suppresses allergen-induced cutaneous late responses  Giuseppina Rotiroti, MD, Mohamed Shamji, PhD, Stephen.
The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy  Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy  Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.
A randomized, double-blind, placebo-controlled pilot study of sublingual versus oral immunotherapy for the treatment of peanut allergy  Satya D. Narisety,

A randomized, double-blind, placebo-controlled pilot study of sublingual versus oral immunotherapy for the treatment of peanut allergy  Satya D. Narisety,
Therapeutic reversal of food allergen sensitivity by mature retinoic acid–differentiated dendritic cell induction of LAG3+CD49b−Foxp3− regulatory T cells 

Therapeutic reversal of food allergen sensitivity by mature retinoic acid–differentiated dendritic cell induction of LAG3+CD49b−Foxp3− regulatory T cells 
Selective ablation of mast cells or basophils reduces peanut-induced anaphylaxis in mice  Laurent L. Reber, PhD, Thomas Marichal, DVM, PhD, Kaori Mukai,

Selective ablation of mast cells or basophils reduces peanut-induced anaphylaxis in mice  Laurent L. Reber, PhD, Thomas Marichal, DVM, PhD, Kaori Mukai,
IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling  Clinton B. Mathias, PhD, Suejy.

IgE-mediated systemic anaphylaxis and impaired tolerance to food antigens in mice with enhanced IL-4 receptor signaling  Clinton B. Mathias, PhD, Suejy.
Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A

Jay A. Lieberman, MD, Faith R. Huang, MD, Hugh A
Odelya E. Pagovich, MD, Bo Wang, MD, Maria J

Odelya E. Pagovich, MD, Bo Wang, MD, Maria J
Maternal allergy increases susceptibility to offspring allergy in association with TH2- biased epigenetic alterations in a mouse model of peanut allergy 

Maternal allergy increases susceptibility to offspring allergy in association with TH2- biased epigenetic alterations in a mouse model of peanut allergy 
Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.
Allergic sensitization can be induced via multiple physiologic routes in an adjuvant- dependent manner  David Dunkin, MD, M. Cecilia Berin, PhD, Lloyd.

Allergic sensitization can be induced via multiple physiologic routes in an adjuvant- dependent manner  David Dunkin, MD, M. Cecilia Berin, PhD, Lloyd.
Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,

Oral immunotherapy induces IgG antibodies that act through FcγRIIb to suppress IgE- mediated hypersensitivity  Oliver T. Burton, PhD, Stephanie L. Logsdon,
Ramipril and metoprolol intake aggravate human and murine anaphylaxis: Evidence for direct mast cell priming  Maria Nassiri, MS, Magda Babina, PhD, Sabine.

Ramipril and metoprolol intake aggravate human and murine anaphylaxis: Evidence for direct mast cell priming  Maria Nassiri, MS, Magda Babina, PhD, Sabine.
Safety, clinical, and immunologic efficacy of a Chinese herbal medicine (Food Allergy Herbal Formula-2) for food allergy  Julie Wang, MD, Stacie M. Jones,

Safety, clinical, and immunologic efficacy of a Chinese herbal medicine (Food Allergy Herbal Formula-2) for food allergy  Julie Wang, MD, Stacie M. Jones,
Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis  Lisa M. Bartnikas, MD, Michael F. Gurish,

Epicutaneous sensitization results in IgE-dependent intestinal mast cell expansion and food-induced anaphylaxis  Lisa M. Bartnikas, MD, Michael F. Gurish,
Matthew C. Tunis, BSc, Wojciech Dawicki, PhD, Kaitlyn R

Matthew C. Tunis, BSc, Wojciech Dawicki, PhD, Kaitlyn R
Odelya E. Pagovich, MD, Bo Wang, MD, Maria J

Odelya E. Pagovich, MD, Bo Wang, MD, Maria J
Forkhead box protein 3 demethylation is associated with tolerance induction in peanut- induced intestinal allergy  Meiqin Wang, MD, PhD, Ivana V. Yang,

Forkhead box protein 3 demethylation is associated with tolerance induction in peanut- induced intestinal allergy  Meiqin Wang, MD, PhD, Ivana V. Yang,

Similar presentations

Ads by Google