1. Grading of smears examined in ordinary microscopy at 1000X magnification after ZN staining
Exercise: Quantification of AFB in ZN Smears

2. ----- smear contains no AFB in 100 fields.
NEG

3. How will you grade a smear with 9 AFB in 100 fields ?
positive(Actual)
09 AFB

4. ------ Smear with more than 10 AFB per field.
3 +

5. A smear with 1 to 9 bacilli in majority of the fields will be graded as:
2 +

6. (1-9 AFB /field in at least 50 fields)
Smear grade: 2 +
(1-9 AFB /field in at least 50 fields)
Exercise: Quantification of AFB in ZN Smears

7. 10 - 99 AFB in 100 fields Smear Grade: 1 +
Exercise: Quantification of AFB in ZN Smears

8. Smear Grade:actual ( < 10 AFB in 100 fields)
Exercise: Quantification of AFB in ZN Smears

9. Grade the following smears:
Exercise: Quantification of AFB in ZN Smears

10. Exercise: Quantification of AFB in ZN Smears

11. Positive (6AFB)
Exercise: Quantification of AFB in ZN Smears

12. Exercise: Quantification of AFB in ZN Smears

13. Positive 1+
Exercise: Quantification of AFB in ZN Smears

14. Exercise: Quantification of AFB in ZN Smears

15. 1 + (86 AFB /96 HPF)
Exercise: Quantification of AFB in ZN Smears

16. Exercise: Quantification of AFB in ZN Smears

17. 08 AFB (Actual)

18. Exercise: Quantification of AFB in ZN Smears

19. Positive 2 +
Exercise: Quantification of AFB in ZN Smears

20. Exercise: Quantification of AFB in ZN Smears

21. Positive (8 AFB)
Exercise: Quantification of AFB in ZN Smears

22. Exercise: Quantification of AFB in ZN Smears

23. 2 +

24. Exercise: Quantification of AFB in ZN Smears

25. Positive 1 +
Exercise: Quantification of AFB in ZN Smears

26. Credit
Revised National Tuberculosis Control Program-Tuberculosis Research Center
Chennai, India