1. Introduction Glycolysis
Bacterial Catabolism
Introduction
Glycolysis

2. Bacterial Catabolism Introduction
Catabolic activities include the break down of glucose to release energy in form of ATP
Reactions include three pathways
Glycolysis
Krebs’ Cycle
Oxidative Phosphorylation (Electron Transport)
Alternative pathways

3. Bacterial Catabolism Glycolysis - Includes: Activation Oxidation
ATP Synthesis
NADH2 Production

4. Bacterial Catabolism Glycolysis “splitting” of sugar
Glucose to pyruvate
Substrate phosphorylation
10 reactions / two phases
Activation
Energy Production
Evolution - probably oldest

5. Bacterial Catabolism

6. Bacterial Catabolism
Activation of glucose by ATP

7. Bacterial Catabolism
Activate with second ATP

8. Bacterial Catabolism
Split sugar into 2 x 3C molecules

9. Bacterial Catabolism
Oxidation and phosphate addition

10. Bacterial Catabolism
ATP made at substrate level from 1,3-PGA

11. Bacterial Catabolism

12. Bacterial Catabolism

13. Bacterial Catabolism
Second ATP made from PEP

14. Bacterial Catabolism Summary of glycolysis 1 Glucose to 2 pyruvate
2 NADH2 produced
2 ATP consumed
2 ATP for each 3C molecule produced
Net yield of 2 ATP

15. Bacterial Catabolism Anaerobic Fermentation
NADH2 must be recycled for glycolysis to continue to produce ATP
Pyruvate to lactate
Pyruvate to ethanol & CO2
Pyruvate to propionate

16. Bacterial Catabolism

17. Bacterial Catabolism