1. Follicular fluid and mural granulosa cells microRNA profiles vary in in vitro fertilization patients depending on their age and oocyte maturation stage 
Juan Manuel Moreno, Ph.D., María José Núñez, M.D., Alicia Quiñonero, Sebastian Martínez, Marina de la Orden, Ph.D., Carlos Simón, M.D., Antonio Pellicer, M.D., César Díaz-García, M.D., Francisco Domínguez, Ph.D. 
Fertility and Sterility 
Volume 104, Issue 4, Pages e1 (October 2015)
DOI: /j.fertnstert
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
(A) Differential expression of FF miRNA according to age. RT-qPCR of hsa-miR-424 in FF groups from 12 YA (<35 year old) and 14 AA (>37 year old) patients. (B) MiRNA profile differences in FF from GV and MII oocytes. (C) RT-qPCR for hsa-miR-451 in the paired (same patient) FF of MII and GV oocytes (n = 9). (D) RT-qPCR for hsa-miR-563 in the paired (same patient) FF of MII and GV oocytes (n = 9). (E) Unsupervised PCA of microarray pools from the GV and MII groups. (F) RT-qPCR for hsa-miR-451 in paired (same patient) GCs from MII and GV oocytes (n = 9). (G) RT-qPCR of hsa-miR-563 in paired (same patient) GCs from MII and GV oocytes (n = 9).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
(A) MiRNA profile differences in FF from MI and MII oocytes. (B) RT-qPCR for hsa-miR-451 in the paired (same patient) FF from MII and MI oocytes (n = 6). (C) RT-qPCR for hsa-miR-547 in the paired (same patient) FF from MII and MI oocytes (n = 6). (D) Unsupervised PCA of microarray pools from the MI and MII groups. (E) RT-qPCR for hsa-miR-451 in paired (same patient) GCs from MII and MI oocytes (n = 8). (F) RT-qPCR for hsa-miR-547 in paired (same patient) GCs from MII and MI oocytes (n = 8).
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
(A) DIANA-miRPath results for the most represented KEGG pathways for in silico miRNA-targeted genes for GV versus MII and MI versus MII studies. (B) Summary of genes targeted by miRNAs from the GnRH signaling pathway.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 1
Study design divided into two stages: the miRNA profile in FF from different oocyte maturation stages and the miRNA profile in MII oocyte FF from different age patients.
Fertility and Sterility  , e1DOI: ( /j.fertnstert )
Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions