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Shaoyu Zhou, Keyaunoosh Kassauei, David J. Cutler, Giulia C

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Presentation on theme: "Shaoyu Zhou, Keyaunoosh Kassauei, David J. Cutler, Giulia C"— Presentation transcript:

1 An Oligonucleotide Microarray for High-Throughput Sequencing of the Mitochondrial Genome 
Shaoyu Zhou, Keyaunoosh Kassauei, David J. Cutler, Giulia C. Kennedy, David Sidransky, Anirban Maitra, Joseph Califano  The Journal of Molecular Diagnostics  Volume 8, Issue 4, Pages (September 2006) DOI: /jmoldx Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Comparison of detection of mitochondrial DNA variations by v1.0 and v2.0 MitoChips. A total of 14 tumor samples were sequenced with v1.0 and v2.0 chips, respectively. Variations were obtained by comparing the sequencing results to mitochondrial DNA reference from MitoMap database. The variations for coding region and D-loop part are presented separately. The Journal of Molecular Diagnostics 2006 8, DOI: ( /jmoldx ) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Chromatograms showing conventional sequencing of D-loop in representative samples. Arrows indicate the variation sites identified in MitoChips. The corresponding sequencing results of MitoChip and conventional assay are presented to the left of the corresponding chromatograms. Nucleotide sites are specified in parentheses. The Journal of Molecular Diagnostics 2006 8, DOI: ( /jmoldx ) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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