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Comparative assessment of five serum antimüllerian hormone assays for the diagnosis of polycystic ovary syndrome Pascal Pigny, Ph.D., Elisse Gorisse, M.D., Amjad Ghulam, M.D., Geoffroy Robin, M.D., Sophie Catteau-Jonard, M.D., Ph.D., Alain Duhamel, M.D., Ph.D., Didier Dewailly, M.D. Fertility and Sterility Volume 105, Issue 4, Pages e3 (April 2016) DOI: /j.fertnstert Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 (A) Correlation between Gen II ELISA, AL-105i ELISA, or Access Dxi automatic assay and EIA AMH/MIS ELISA was evaluated by the Passing-Bablok regression procedure. The blue line shows the regression curve; the dashed red lines show the 95% CI around the regression curve; the pink line shows the line of perfect correlation. (B) Bland-Altman plots showing the distribution of the differences of AMH concentration between two methods (y-axis) according to the average of AMH concentration (x-axis). The red horizontal line shows the mean difference (or bias). The dashed red lines show the 95% CI around the mean difference. AMH values are expressed in nanograms per milliliter; 1 ng/mL corresponds to 7.14 pmol/L. Fertility and Sterility , e3DOI: ( /j.fertnstert ) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions
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Figure 2 Box and whisker plots showing the values of serum AMH in nanograms per milliliter (y-axis, log scale) with each assay in the two subgroups of patients: controls and PCOS patients with a full phenotype. Horizontal small bars represent the 5th to 95th percentile range, and the boxes indicate the 25th to 75th percentile range. The horizontal line in each box corresponds to the median; 1 ng/mL corresponds to 7.14 pmol/L. Fertility and Sterility , e3DOI: ( /j.fertnstert ) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions
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Supplemental Figure 1 (A) Correlation between different AMH immunoassays was evaluated by Passing and Bablok regression procedure. Blue line, Regression curve. Dashed red lines, 95% confidence interval (CI) around the regression curve. Pink line, line of perfect correlation. (B) Bland-Altman plots showing the distribution of the differences of AMH concentration between 2 methods (Y axis) according to the average of AMH concentration (X axis). Red horizontal line, Mean difference (or bias). Dashed red lines, 95% CI around the mean difference. AMH values are expressed in ng/ml; 1 ng/ml corresponds to 7.14 pmol/L. Fertility and Sterility , e3DOI: ( /j.fertnstert ) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions
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Supplemental Figure 2 Repartition of controls between groups 1A (true controls) and 1B (controls with PCOM) according to cluster analysis run with each assay-specific AMH values. Each square represents one control woman. Squares are ranked according to AMH values obtained with the EIA AMH/MIS assay. Fertility and Sterility , e3DOI: ( /j.fertnstert ) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions
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Supplemental Figure 3 Box and whisker plots showing the values of serum AMH in ng/ml (Y axis, log scale) with each assay in the 3 subgroups of patients: 1A: “true” controls (white boxes); 1B: controls with PCOM (green boxes); PCOS: patients with a classic PCOS phenotype. Horizontal small bars represent the 5–95 percentile range, and the boxes indicate the 25–75 percentile range. The horizontal line in each box corresponds to the median. The colored horizontal lines indicate the proposed upper limit of normal values of serum AMH obtained with ELISAs (green line) or fully automated assays (red line). Fertility and Sterility , e3DOI: ( /j.fertnstert ) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions
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