1. Characterization of cassava Germplasm core collection in
THAILAND
Prapit Wongtiem

2. Rayong Field Crops Research (RYFCRC), Department of Agriculture (DOA)

3. The cassava germplasm collection in Thailand
Rayong Field Crops Research (RYFCRC),
Department of Agriculture (DOA) collected the varieties
by ourselves more than 200 accessions

4. collected the varieties by ourselves (279)
Table 1. The cassava germplasm collection in Rayong Field Crops Research (RYFCRC)
collected the varieties by ourselves (279)
Sources
No. accessions
Scientific names
Local Manihot esculenta Grantz.
Wild M. giaziovii
Introduce
Virgin Island 17 M. esculenta
Indonesia 5 M. esculenta
Vietnam 6 M. esculenta
China M. esculenta
Laos M. esculenta
CIAT 48 M. esculenta
Promising M. esculenta

5. In vitro conservation 2001,CIAT, Colombia 600 accessions.
RYFCRC collect and conserve in 2 conditions
1. In the laboratory, subculture every 3-4 months
2. In the field condition

6. Table 2. Cassava germplasm core collection from CIAT
variental
name
# accessions
variental
name
# accessions
variental
name
# accessions CG CM CR
MARG
MBOL
MBRA
MCHN
MCOL
MCUB 12 18 21 8 3 99 2
160
MDOM
MECU
MFJI
MGUA
MHMC
MIND
MMAL
MMEX
MNGA 3 30 2 15 1 7 19
MPAN
MPAR
MPER
MPHI
MPTR
MTAI
MUSA
MVEN SG 9 39 73 2 6 4 52
Total 628 accessions

7. Propagation by tissue culture
Subculture
0-7 days
1.5-2 months
3 months

8. Transfer to green house
At room temperature at 1 week
Coconut fifer

9. 25-30 days

10. Field Collection
1.5 months
3-4 months
12 months

11. Characterization of cassava germplasm
core collection

12. Descriptors to be scored at 3 months after planting
Color of Apical Leaves

13. Record the most frequent occurrence
Color of Apical Leaves
Record the most frequent occurrence

14. Color of Apical Leaves

15. Descriptors to be scored at 6 months and 9 months after planting
- Petiole color Color of leaf vein
- Leaf color Color of stem cortex
- Number of leaf lobes - Color of stem epidermis
- Length of leaf lobe - Color of stem exterior
- Width of leaf lobe - Distance between leaf scars
- Lobe margins - Growth habit of stem
- Petiole length

16. Record the most frequent occurrence
Dark purple
purple
red
Record the most frequent occurrence
green
Petiole Color
Redish-green
Greenish-purple

17. Orientation of petiole
Observe from the middle of the plant
Inclined upwards
Horizontal
Irregular
Inclined downwards

18. Descriptors to be scored at 6 months after planting Leaf Color
A leaf from the middle of the plant
Record the most frequent occurrence
purple
Purple-green
Green-purple
Green -white
Light green
Dark green

19. Record the most frequent occurrence
straight
Oblong-lanceolate
ovoid
Obovate-lanceolate
Shape of central leaflet
lanceolate
Elliptic-lanceolate
lanceolate

20. red
pink
Reddish white
white
Color of leaf vein

21. Descriptors to be scored at 6 months after planting
Number of Leaf Lobes
3 lobes
5 lobes
7 lobes
7 lobes
9 lobes
Observe a leaf from the middle of the plant ( 5 leaves )

22. Descriptors to be scored at 6 months after planting
Length and Width of Leaf Lobe
Measure 3 leaves from the middle of the plant ( cm )

23. Descriptors to be scored at 6 months after planting
Petiole Length
Short
(≤15cm.)
Medium
(16-25cm.)
Long
(>25cm.)
Observe from the middle of the plant

24. Descriptors to be scored at 6 months after planting
Flowering
Absent Present

25. Descriptors to be scored at 9 months after planting
Distance between leaf scars
Measure from the middle of stem and plant ( cm )
Divide the distance by the number of node
Short
Medium
Long

26. Color of stem exterior observe on the middle of the plant ( cm )

27. Growth habit of stem
Zig-zag
Straight

28. The Descriptors to be scored at harvest such as
- Plant height
- Height to first branching
- Levels of branching
- Branching habit
- Angle of branching
- Dry matter content
- Starch content
- Harvest index

29. 120 CM
310 CM
Level of Branching

30. Plant height Height to first branching
Record measurements from 3 plants

31. No branch
Branch and angle

32. Angle of branching- measure at first primary branching
From 3 plants

33. Fruit
Absent Present

34. Record the root from three plant
Dark brown
yellow
External color of storage root
Record the most frequent occurrence
Number of storage
roots/plant
Record the root from three plant
Light brown
cream

35. Extent of root peduncle
Pedunculate
Sessile

36. Color of root cortex
Record the most frequent occurrence

37. Color of root pulp (parenchyma)
Record the most frequent occurrence

38. Starch content (%)

39. Table 3 The Morphological for characterization the
Cassava core germplasm
Character Min Max Mean
Plant ht.(cm)
Stem/Plant
Leaf lobes/Leaf
Root/Plant
Root wt.(Kg/Plant)
% Starch

40. clone root/plant % starch root wt.(t./ha)
Table 4 Yield (t/ha) : The cassava germplasm core
collection in RYFCRC
clone root/plant % starch root wt.(t./ha) CM
MVEN 90 CM
MCOL 2215
MMEX 8
MCOL 2638
MCOL 1805
MCOL 1516
Rayong 7
Rayong 9 6 10 8 7 11 13 31 30 29 28 32
39.38
25.82
25.69
30.69
32.69
26.63
25.88
38.44
38,00

41. Acknowleagements CIAT, Dr. Daniel Debouck Dr. Hernan Ceballos
Dr. Reinhardt Hoewler
Dr. Martin Fregene
Rayong Field Crops Research center (RYFCRC)
Department of Agriculture (DOA) , Thiland

42. Thank you
for your
listening