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Cutaneous, But Not Airway, Latex Exposure Induces Allergic Lung Inflammation and Airway Hyperreactivity in Mice  Maili Lehto, Rita Haapakoski, Henrik.

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Presentation on theme: "Cutaneous, But Not Airway, Latex Exposure Induces Allergic Lung Inflammation and Airway Hyperreactivity in Mice  Maili Lehto, Rita Haapakoski, Henrik."— Presentation transcript:

1 Cutaneous, But Not Airway, Latex Exposure Induces Allergic Lung Inflammation and Airway Hyperreactivity in Mice  Maili Lehto, Rita Haapakoski, Henrik Wolff, Marja-Leena Majuri, Mika J. Mäkelä, Marina Leino, Timo Reunala, Kristiina Turjanmaa, Timo Palosuo, Harri Alenius  Journal of Investigative Dermatology  Volume 125, Issue 5, Pages (November 2005) DOI: /j X x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Lung inflammation. (A) Inflammation was expressed as score values and eosinophils as percentages of inflammatory cells (n=7–12 mice per group). (B) Lymphocytes and eosinophils in bronchoalveolar lavage (BAL) fluids were counted per high-power field (n=12–16 mice per group). The columns and error bars represent mean±SEM; *p<0.05, **p<0.01, ***p<0.001. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Airway hyperresponsiveness and lung mucus production. (A) Airway hyperreactivity was expressed by average enhanced pause (Penh) values in relation to increasing doses of aerosolized methacholine. Data represents mean±SEM. (B) The number of mucus-producing cells was represented as periodic acid-Schiff (PAS)-positive cells. The columns and error bars represent mean±SEM; ns, non-significant, *p<0.05, **p<0.01, ***p<0.001; n=12–16 mice per group. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Chemokine mRNA exposure. mRNA expression of chemokines, which attract especially eosinophils (A) or T-helper type 2 cells (B) in lung samples after exposures to vehicle (saline (SAL)) or natural rubber latex (NRL). Relative units (RU) are relative differences compared with the calibrator. The columns and error bars represent mean±SEM; ns, non-significant, *p<0.05, **p<0.01, ***p<0.001; n=12–15 mice per group. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Expression levels of cytokines and Foxp3 mRNAs. T-helper type 2 cytokine mRNA (A) and regulatory cytokine and forkhead box 3 (Foxp3) mRNA (B) expression in lung samples after exposures to vehicle (saline (SAL)) or natural rubber latex (NRL). The columns and error bars represent mean±SEM; ns, non-significant, *p<0.05, **p<0.01, ***p<0.001; n=12–15 mice per group. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Immunoglobulin E (IgE) levels. Total serum and prohevein (Hev b 6.01)-specific IgE levels in mice after exposures to vehicle (saline (SAL)) or natural rubber latex (NRL) were measured by ELISA. Specific IgE levels of the serum are expressed as absorbance units (405 nm). The columns and error bars represent mean±SEM; *p<0.05, **p<0.01, ***p<0.001; n=12–15 mice per group. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Intracutaneous (IC), intraperitoneal (IP), and intranasal (IN) exposure schedules. Mice were exposed for 4 wk before airway challenge. In saline (SAL) groups exposures were made with phosphate-buffered saline and with natural rubber latex (NRL) in NRL groups. Airway challenges were made by NRL. Airway hyperreactivity to methacholine was measured 24 h after airway challenges and different samples were taken for subsequent analysis. Journal of Investigative Dermatology  , DOI: ( /j X x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

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