1. Characterization of lactoferrin receptor on human spermatozoa
Peng Wang, Bianjiang Liu, Zengjun Wang, Xiaobing Niu, Shifeng Su, Wei Zhang, Xinru Wang 
Reproductive BioMedicine Online 
Volume 22, Issue 2, Pages (February 2011)
DOI: /j.rbmo
Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Amplification of human lactoferrin receptor (LFR) gene from a human testis cDNA library and expression of recombinant LFR protein. (A) LFR-amplified products showed by agarose gel electrophoresis, M=DNA marker. (B) Recombinant LFR protein probed by anti-LFR antibody via Western blot analysis.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Phosphatidylinositol-specific phospholipase C treated sperm-surface proteins analysed via Western blot. Lanes 1 and 2, sperm-surface protein extract probed with anti-lactoferrin receptor (LFR) antibody under reduced and non-reduced conditions, respectively; lanes 3 and 4, protein extract probed with anti-LFR antibody pre-absorbed with recombinant LFR under reduced and non-reduced conditions, respectively; lane 5, protein extract probed with anti-lactoferrin (LF) antibody; lane 6, protein extract probed with anti-LF antibody pre-absorbed with human LF protein.
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4. Figure 3
Lactoferrin (LF) binding to recombinant lactoferrin receptor (LFR) analysed via Far-Western blot. Lane 1, pre-immobilized rLFR incubated with LF for 1h, washed and probed with anti-LF; lane 2, pre-immobilized rLFR incubated without LF for 1h, washed and probed with anti-LF; lane 3, pre-immobilized LF incubated with rLFR for 1h, washed and probed with anti-LFR; lane 4, pre-immobilized LF incubated without rLFR for 1h, washed and probed with anti-LFR.
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5. Figure 4
Lactoferrin (LF) binding to recombinant lactoferrin receptor (LFR) analysed via immunoprecipitation. Lane 1, immunoprecipitation complex was separated and stained (72kDa=LF, 34kDa=recombinant LFR, 26 and 48kDa=anti-LF antibody); lane 2, anti-LF antibody stained as control; lane 3, immunoprecipitation complex was probed with anti-LFR antibody.
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6. Figure 5
Binding assay of lactoferrin (LF) to recombinant lactoferrin receptor (LFR) via autoradiography analysis. Lanes 1 and 2, protein stain of recombinant LFR; lane 3, lane 1 probed with 125I-LF; lane 4, lane 2 probed with 125I-LF under the competition of 10× unlabelled LF.
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7. Figure 6
Saturation binding of 125I-labelled lactoferrin (LF) to recombinant lactoferrin receptor (rLFR). (A) Increasing amounts of 125I–LF were incubated with rLFR (♦) or without rLFR (●). (B) displacement of 125I–LF from rLFR with increasing amounts of unlabelled LF (♦). Each point represents mean±SD of three experiments.
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8. Figure 7
Immunofluorescent localization of lactoferrin receptor (LFR) on human spermatozoa. (A) Spermatozoa were probed with anti-LFR antibody. (B) Control experiments were performed with normal mouse serum. Bars=10μm.
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