1. Effects of retinoic acid on the inner cell mass in mouse blastocysts
Fu-Jen Huang, M.D., Yu-Cheng Hsu, B.Sc., Hong-Yo Kang, Ph.D., Shiuh-Young Chang, M.D., Yan-Der Hsuuw, Ph.D., Ko-En Huang, M.D. 
Fertility and Sterility 
Volume 83, Issue 1, Pages (January 2005)
DOI: /j.fertnstert
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
(A, B) Identification of DNA strand breaks by the TUNEL method. (A) The TUNEL-negative cells (arrow) in control ICMs are visualized under the visible-light microscope and are stained light brown. (B) The TUNEL-positive cells in the RA-treated ICMs (arrowhead) are stained black. The TUNEL-negative cells (arrow) are stained brown. (C, D) Identification of early apoptosis and necrosis by the Annexin-V-FLUOS staining method. (C) The Annexin-V-negative cells in control ICMs are colorless under the fluorescence microscope. (D) The necrotic cells (arrowhead) in the RA-treated ICM are stained orange-red. The apoptotic cells (arrow) in the ICM are stained green. (E, F) Cell-count staining in the ICM. (E) The ICM cells are stained blue. (F) The ICM cells are spread out and easily counted after the slide-cover pressing. Original magnification, ×400.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions