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IL-5 receptor α levels in patients with marked eosinophilia or mastocytosis  Todd M. Wilson, DO, Irina Maric, MD, Juhi Shukla, MSc, Margaret Brown, BS,

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Presentation on theme: "IL-5 receptor α levels in patients with marked eosinophilia or mastocytosis  Todd M. Wilson, DO, Irina Maric, MD, Juhi Shukla, MSc, Margaret Brown, BS,"— Presentation transcript:

1 IL-5 receptor α levels in patients with marked eosinophilia or mastocytosis 
Todd M. Wilson, DO, Irina Maric, MD, Juhi Shukla, MSc, Margaret Brown, BS, Carlo Santos, MSc, Olga Simakova, PhD, Paneez Khoury, MD, Michael P. Fay, PhD, Alexander Kozhich, PhD, Roland Kolbeck, PhD, Dean D. Metcalfe, MD, Amy D. Klion, MD  Journal of Allergy and Clinical Immunology  Volume 128, Issue 5, Pages e3 (November 2011) DOI: /j.jaci Copyright © Terms and Conditions

2 Fig 1 Serum levels of sIL-5Rα are positively correlated with peripheral blood EO. Each circle represents an individual data point. Subjects with eosinophilic disease are shown in red, those with mastocytosis in blue, and normals in green. The dotted line represents the limit of detection of the assay. r = 0.54; P < .0001; Spearman rank correlation. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © Terms and Conditions

3 Fig 2 Serum IL-5Rα levels are increased in patients with EO and mastocytosis without EO. Each symbol represents an individual data point. Subjects with EO are indicated in red, with mastocytosis in blue, and normal controls in green. Group GMs are indicated by horizontal lines. ∗P < .001 or †P < .05 compared with normal group (Holm’s adjusted P value using the Mann-Whitney U test). Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © Terms and Conditions

4 Fig 3 Surface expression of IL-5Rα on eosinophils is inversely correlated with peripheral blood EO. Each circle represents an individual data point. Subjects with eosinophilic disease are shown in red, those with mastocytosis in blue, and normal controls in green. ABC, Antibodies bound per cell. r = −0.48; P < .0001; Spearman rank correlation. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © Terms and Conditions

5 Fig 4 Serum levels of sIL-5Rα are correlated with serum tryptase levels in patients with SM, but not HES without SM. Serum tryptase and sIL-5Rα levels are shown for subjects with SM (r = 0.62; P < .0001; Spearman rank correlation, panel A) and EO without SM (r = 0.19; P = .2; panel B). Each circle represents an individual data point. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © Terms and Conditions

6 Fig E1 Bone marrow mast cells express IL-5Rα on their surface. A representative histogram depicts surface expression of IL-5Rα on bone marrow mast cells (left panel) and eosinophils (middle panel) from a patient with KIT D816V-positive SM. IL-5Rα surface expression was not detected on the patient’s bone marrow lymphocytes (right panel). The area under the curve for the isotype control (IgG-PE) is indicated in gray and for IL-5Rα (CDw125-PE) in white. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © Terms and Conditions

7 Fig E2 Human bone marrow mast cells express mRNA for sIL5Rα. Total RNA was isolated from human fibroblasts (ATCC cell line CCL-202), flow-sorted human bone marrow mast cells from a patient with SM, and purified human peripheral blood eosinophils reverse-transcribed and amplified using primers specific for sIL5Rα and β-actin as described in the Methods section. The PCR products were run on a 2% agarose gel and visualized with ethidium bromide. NTC, No template control. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © Terms and Conditions

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