1. Protosensor‐mediated detection of pathological glycosuria in patient clinical samples
Protosensor‐mediated detection of pathological glycosuria in patient clinical samples Flow cytometry evaluation of protosensors structural robustness in urines. GluONe protosensors were induced and incubated for 2 h at 25°C in human urine and analyzed using flow cytometry while recording Resorufin fluorescence and forward‐scattered light.Left: Dot histogram of data used for statistical analysis. Dots of the same color (white, gray, black) correspond to one repetition of the experiment, which was performed in triplicates in different patient samples. Above each data point is depicted a photograph of a urinary dipstick measurement performed on the sample. We then plotted a dot histogram of data comparing the non‐diabetic to diabetic set. Right: Receiver operating characteristic (ROC) analysis curve depicting statistic sensitivity versus (1‐specificity). A set of 72 measurement performed in non‐pathological urine were compared to 72 measurements performed in urine from diabetic patients. The data were processed using the software SigmaPlot (Systat Software Inc.) with a computed P <  using a Mann–Whitney–Wilcoxon test. We used GluONe protosensors for this assay and measured Out1 signal (NADH absorbance) after 60 min at room temperature.
Alexis Courbet et al. Mol Syst Biol 2018;14:e7845
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