1. Prmt7 deficiency causes a fiber-type switch toward glycolytic.
Prmt7 deficiency causes a fiber-type switch toward glycolytic. A: qRT-PCR of TA muscles from 4-month-old Prmt7+/+ and Prmt7−/− mice for different Myh types (n = 5 per group). Data represent means ± SEM. *P < 0.05, **P < B: Immunostaining of 4-month-old Prmt7+/+ and Prmt7−/− TA muscles for MyhIIa and MyhIIb. Scale bars, 100 μm. C: Quantification of the cross-sectional area (CSA) of MyhIIa- or MyhIib-positive myofibers in TA muscles (n = 3). Data represent means ± SEM. *P < 0.05, **P < 0.01, ***P < D: Histochemical staining of NADH-TR and SDH enzymatic activity in 4-month-old TA and EDL muscles (n = 5 per group). Scale bar, 100 μm. E: Quantification of stained myofibers by 3 different staining grades (n = 5). Data represent means ± SEM. ***P < 0.001, **P < 0.01, *P < ns, not significant.
Hyeon-Ju Jeong et al. Diabetes 2016;65:
©2016 by American Diabetes Association