Presentation is loading. Please wait.

Presentation is loading. Please wait.

D. Z. Xiang, MD, E. K. Verbeken, MD, PhD, A. T. L. Van Lommel, PhD, M

Published byNathaniel Allison Modified over 6 years ago

Similar presentations

Presentation on theme: "D. Z. Xiang, MD, E. K. Verbeken, MD, PhD, A. T. L. Van Lommel, PhD, M"— Presentation transcript:

1 Composition and formation of the sleeve enveloping a central venous catheter 
D.Z. Xiang, MD, E.K. Verbeken, MD, PhD, A.T.L. Van Lommel, PhD, M. Stas, MD, I. De Wever, MD, PhD  Journal of Vascular Surgery  Volume 28, Issue 2, Pages (August 1998) DOI: /S (98) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Day 3: Some smooth muscle cells in the vein wall close to the catheter show signs of injury: membrane disruption (solid triangle), cytoplasmic debris and autophagocytotic bodies (arrow), pycnotic nuclei (open triangle)(TEM 12600×). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 A, Day 1: A pericatheter thrombus (PCT) is present between the vein wall (V) and the catheter mold (C) (original magnification, 25×; HE stain). B, The pericatheter thrombus is negative on Masson's trichrome stain (original magnification, 25×; Trichrome stain). C, In the vein wall (V), a thin continuous layer of α-actin positive cells represents the muscle layer. No α-actin stain can be observed in the pericatheter thrombus (PCT) (original magnification, 25×; α-actin stain). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 A, Day 7: The former pericatheter thrombus is populated by numerous cells and can be called a catheter sleeve (CS) (V = vein wall; C = catheter) (original magnification, 25×; HE stain). B, Masson's trichrome stain in the sleeve (CS) is more intense near the vein wall (V) than near the catheter mold (C). In some areas negative on Masson's trichrome stain, almost no α-actin stain is visible (arrow)(original magnification, 400×; Trichrome stain). C, α-actin positive cells are more intense in the zone of the sleeve near the vein wall (arrow) than in the zone near the catheter mold (star) (original magnification, 25×; α-actin stain). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Day 7: The α-actin positive cells are distributed in a stream-like pattern from the vein wall (V) along the adherent areas (star) into the catheter sleeve (CS). Cell density is higher in the vicinity of the vessel wall and decreases towards the catheter mold (C) (original magnification 100×; α-actin stain) Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 Day 7: Several connecting bridges (B) divide the barium-filled space (star) between the vein wall (V) and the catheter sleeve (CS). These tiny bridges, filled with α-actin positive cells, indicate the migratory pathway of the smooth muscle cells (original magnification, 200×; α-actin stain). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 6 Day 7: This overview picture of the sleeve shows a layer of endothelial cells (EC) at the luminal side, several layers of smooth muscle cells of the synthetic phenotype (star) with collagen (0) in between, and still some fibrin (F) at the catheter side(TEM, 1440×). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 7 A, Day 7: A smooth muscle cell (arrow) migrates from the vein wall (V) into the bridge (B) to the catheter sleeve (CS). (TEM, 2610×). B, Higher magnification of the cell indicated in A shows its synthetic phenotype: large amounts of rough endoplasmatic reticulum, free ribosomes, and only a few filament bundles. The pseudopodia (triangle) are the hallmark of migration (TEM, 12600×). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

9 Fig. 8 Day 14: The SEM image taken after 14 days of catheterization shows that the bridges (B) between the vein wall (V) and the catheter sleeve (CS) and the surface of the sleeve are covered by nicely oriented endothelial cells (arrow)(SEM, 300×). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

10 Fig. 9 The distribution curve of catheter sleeve (CS) and sleeve remnant (SR). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

11 Fig. 10 Ten months after withdrawal of the catheter, the remnant of the collapsed sleeve (R) is easily recognized by the clot (triangle) in its center, and the bridges (star) linking it with the vein wall (V) (original magnification, 100×; toluidine blue stain). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Download ppt "D. Z. Xiang, MD, E. K. Verbeken, MD, PhD, A. T. L. Van Lommel, PhD, M"

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.
The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.
Natural history, duplex characteristics, and histopathologic correlation of arterial injuries in a canine model  Thomas F. Panetta, MD, Clifford M. Sales,

Natural history, duplex characteristics, and histopathologic correlation of arterial injuries in a canine model  Thomas F. Panetta, MD, Clifford M. Sales,
Age of onset, pattern of distribution, and histology of aneurysm development in a genetically predisposed mouse model  Colleen M. Brophy, MD, Joan E.

Age of onset, pattern of distribution, and histology of aneurysm development in a genetically predisposed mouse model  Colleen M. Brophy, MD, Joan E.
Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin  Todd K. Rosengart, MD, Kurt.

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin  Todd K. Rosengart, MD, Kurt.
Eddie L. Hoover, M. D. , Margery Ross, Ph. D. , Kazam Fani, M. D

Eddie L. Hoover, M. D. , Margery Ross, Ph. D. , Kazam Fani, M. D
Antisense oligonucleotide to proto-oncogene c-myb inhibits the formation of intimal hyperplasia in experimental vein grafts  Gregory J. Fulton, MB, FRCSI,

Antisense oligonucleotide to proto-oncogene c-myb inhibits the formation of intimal hyperplasia in experimental vein grafts  Gregory J. Fulton, MB, FRCSI,
Septic emboli from a radial artery catheter with local manifestations of subacute bacterial endocarditis  Amihai Shinfeld, MD, Amos Ofer, MD, I. Engleberg,

Septic emboli from a radial artery catheter with local manifestations of subacute bacterial endocarditis  Amihai Shinfeld, MD, Amos Ofer, MD, I. Engleberg,
Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,

Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,
Central venous catheter failure is induced by injury and can be prevented by stabilizing the catheter tip  Ted R. Kohler, MD, Thomas R. Kirkman, BS  Journal.

Central venous catheter failure is induced by injury and can be prevented by stabilizing the catheter tip  Ted R. Kohler, MD, Thomas R. Kirkman, BS  Journal.
Vascular anastomoses with laser energy

Vascular anastomoses with laser energy
Development of a “leak-proof,” knitted Dacron vascular prosthesis

Development of a “leak-proof,” knitted Dacron vascular prosthesis
Atherosclerotic aneurysm formation in an in situ saphenous vein graft

Atherosclerotic aneurysm formation in an in situ saphenous vein graft
Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M

Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M
The role of circulating cells in the healing of vascular prostheses

The role of circulating cells in the healing of vascular prostheses
Vida K. Stark, MS, Thomas F. Warner, MD, John R. Hoch, MD 

Vida K. Stark, MS, Thomas F. Warner, MD, John R. Hoch, MD 
Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material  Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,

Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material  Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,
Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD 
Behavior of vital and killed autologous pericardium in the descending aorta of sheep  David T. Cheung, PhD, Suk Jung Choo, MD, Albert C. Grobe, MS, Douglas.

Behavior of vital and killed autologous pericardium in the descending aorta of sheep  David T. Cheung, PhD, Suk Jung Choo, MD, Albert C. Grobe, MS, Douglas.

Similar presentations

Ads by Google