Presentation is loading. Please wait.

Presentation is loading. Please wait.

Evidence of genotypic diversity among Candida auris isolates by multilocus sequence typing, matrix-assisted laser desorption ionization time-of-flight.

Similar presentations


Presentation on theme: "Evidence of genotypic diversity among Candida auris isolates by multilocus sequence typing, matrix-assisted laser desorption ionization time-of-flight."— Presentation transcript:

1 Evidence of genotypic diversity among Candida auris isolates by multilocus sequence typing, matrix-assisted laser desorption ionization time-of-flight mass spectrometry and amplified fragment length polymorphism  A. Prakash, C. Sharma, A. Singh, P. Kumar Singh, A. Kumar, F. Hagen, N.P. Govender, A.L. Colombo, J.F. Meis, A. Chowdhary  Clinical Microbiology and Infection  Volume 22, Issue 3, Pages 277.e1-277.e9 (March 2016) DOI: /j.cmi Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

2 Fig. 1 Phylogenetic tree based on concatenated sequences of the internal transcribed spacer, RPB1, RPB2 and D1/D2 of Candida auris isolates from India (n = 90), South Africa (n = 6), Brazil (n = 5), Japan (JCM 15448) and Korea (KCTC and KCTC 17810) using neighbour joining phylogenetic analysis with 2000 bootstrap replications using MEGA version 6 software. Clinical Microbiology and Infection  , 277.e1-277.e9DOI: ( /j.cmi ) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

3 Fig. 2 Minimum spanning tree of Candida auris isolates based on amplified fragment length polymorphism fingerprint profiles using permutation analysis, which generated a 100% support value for all branches. Each circle represents one isolate, lines between circles represent relative distance between isolates, colour coding: yellow = India, green = Brazil, brown = South Africa, dark blue = Japan, light blue = South Korea. Thick, regular, thin, thick dashed and regular dashed lines depict relative branch lengths of <10, <20, <30, <40 and ≥40, respectively. Clinical Microbiology and Infection  , 277.e1-277.e9DOI: ( /j.cmi ) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

4 Fig. 3 An unweighted pair group method with averages dendrogram based on main spectrum data using Pearson correlation coefficient generated by Bionumerics software depicting four clusters of Candida auris isolates (n = 104). The isolates are coloured on the basis of their country of origin: yellow = India, green = Brazil, brown = South Africa, dark blue = Japan, light blue = South Korea. Clinical Microbiology and Infection  , 277.e1-277.e9DOI: ( /j.cmi ) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

5 FIG. S1. Phylogenetic tree based on partial internal transcribed spacer sequences of Candida auris from the present study (n = 104) and those from France (n = 1), Kuwait (n = 7) and Malaysia (n = 1), Japan (n = 1) and Korea (n = 2) along with Candida haemulonii (n = 14), C. haemulonii var. vulnera (n = 4), Candida duobushaemulonii (n = 3) and Candida pseudohaemulonii (n = 1) isolates using neighbour-joining analysis with 2000 bootstrap replications. Bootstrap values are shown above the branches. Clinical Microbiology and Infection  , 277.e1-277.e9DOI: ( /j.cmi ) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

6 FIG. S2. Dendrogram of amplified fragment length polymorphism analysis of Candida auris isolates from India (n = 90), South Africa (n = 6), Brazil (n = 5), Japan (n = 1) and South Korea (n = 2). It was constructed by Bionumerics software using unweighted pair group method with averages (UPGMA) in combination with the Pearson correlation coefficient and was restricted to fragments of 60–400 bp. Scale bar indicates the percentage similarity. Clinical Microbiology and Infection  , 277.e1-277.e9DOI: ( /j.cmi ) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions


Download ppt "Evidence of genotypic diversity among Candida auris isolates by multilocus sequence typing, matrix-assisted laser desorption ionization time-of-flight."

Similar presentations


Ads by Google