1. Intracellular noise in the cAMP circuit drives observed population behaviors Firing rate phase diagrams for single cells in a population (top) and the population as a whole (bottom) as predicted by the model with minimal noise (σ = 0.01) as a function of background and firing‐induced cAMP.
Intracellular noise in the cAMP circuit drives observed population behaviors Firing rate phase diagrams for single cells in a population (top) and the population as a whole (bottom) as predicted by the model with minimal noise (σ = 0.01) as a function of background and firing‐induced cAMP. See Supplementary Fig S4 for other noise‐source cases. Example single‐cell (blues) and population (black) cytosolic cAMP traces taken from dual‐expressing Epac1camps/mRFPmars tracer cells for oscillating populations at ˜0.4 ML density, 10 μl/min flow subjected to steps of 10 nM and 500 nM cAMP in microfluidic devices. Mean standard deviations of the single‐cell cytosolic cAMP levels for cells in 10 experimental populations inside microfluidic devices subjected to a step stimulus of cAMP as shown in (B) from 10 to 60 min post‐stimulus. Values are normalized to the mean standard deviation of cells exposed to a 10 nM external cAMP step to show the relative increase in stochastic variability; errors by bootstrapping. Single‐cell cytosolic cAMP responses to eight 1‐nM pulses, 6‐min period with 1‐min‐long pulses (gray) and two 1‐min, two 30‐s, two 20‐s, and two 10‐s pulses (blues) given using microfluidic devices. Source data are available online for this figure.
Allyson E Sgro et al. Mol Syst Biol 2015;11:779
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