Presentation is loading. Please wait.

Presentation is loading. Please wait.

CpG-DNA enhances the tight junction integrity of the bronchial epithelial cell barrier  Terufumi Kubo, MD, PhD, Paulina Wawrzyniak, MSc, Hideaki Morita,

Published byGrace Hamilton Modified over 6 years ago

Similar presentations

Presentation on theme: "CpG-DNA enhances the tight junction integrity of the bronchial epithelial cell barrier  Terufumi Kubo, MD, PhD, Paulina Wawrzyniak, MSc, Hideaki Morita,"— Presentation transcript:

1 CpG-DNA enhances the tight junction integrity of the bronchial epithelial cell barrier 
Terufumi Kubo, MD, PhD, Paulina Wawrzyniak, MSc, Hideaki Morita, MD, PhD, Kazunari Sugita, MD, PhD, Kerstin Wanke, PhD, Jeannette I. Kast, MSc, Can Altunbulakli, MSc, Beate Rückert, Sci Tec, Bogdan Jakiela, PhD, Marek Sanak, MD, PhD, Mübeccel Akdis, MD, PhD, Cezmi A. Akdis, MD  Journal of Allergy and Clinical Immunology  Volume 136, Issue 5, Pages e8 (November 2015) DOI: /j.jaci Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Effect of CpG-2006 stimulation on ALI-cultured bronchial epithelial cells. A, Left panel, Increased TER over time in healthy donor–derived bronchial epithelial cells at different concentrations of CpG-2006 treatment. Right panel, Dose-dependent decrease in FITC-dextran paracellular permeability across nonasthmatic epithelium treated with CpG CpG-2006 was added to cultures in the beginning of the experiment. B, TER (left panel) and paracellular FITC-dextran permeability (right panel) of epithelial cells from nonasthmatic subjects stimulated by IL-13 with or without pretreatment of CpG CpG-2006 or IL-13 was added at t = 0 or t = 24, respectively. TER is compared between the IL-13 plus 10 μmol/L CpG-2006– and IL-13–treated groups. C, TER kinetics (left panel) and paracellular permeability of FITC-dextran (right panel) in bronchial epithelial cells from asthmatic patients in response to 10 μmol/L CpG D, Alteration of gene expression of TJ-related molecules in response to 10 μmol/L CpG Representative data are shown in Fig 1, A-D (n = 3). u.s., Unstimulated. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Immunofluorescence detection of claudin-4 (green) and ZO-1 (red) in ALI-cultured bronchial epithelial cells from nonasthmatic (A) and asthmatic (B) subjects. Representative pictures from 3 donors each are shown. Cells were fixed after 48 hours of stimulation with 10 μmol/L CpG ′,6-diamidino-2-phenylindole (DAPI) was used to visualize nuclei. Bar = 20 μm. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 There is no difference in the efficacy of CpG-2006 treatment between apical and basolateral administration in nonasthmatic bronchial epithelial cells. TER time kinetics (left panel) and paracellular flux (right panel) were measured. CpG-2006 stimulation was started at t = 0 and maintained until finishing the experiment. CpG-2006 treatment of the ALI from apical and basolateral administration versus unstimulated control (u.s.): *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Role of different CpGs and control DNA in the improvement of barrier integrity. Barrier integrity as determined by using TER and paracellular flux in response to CpG-K3 (A), CpG-K23 (B), and control DNA (C) stimulation were determined based on TER (left panels) and FITC-dextran 4-kDa paracelluar flux (right panels), is shown. Stimulations with CpGs or control DNA were started at t = 0 and maintained until finishing the experiments. CpG-K3 and CpG-K23 also enhance barrier integrity. Control DNA does not affect TER or paracellular permeability of FITC-dextran. u.s., Unstimulated. *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 TER kinetics of nonasthmatic bronchial epithelial cells treated with IL-13 and CpG IL-13 treatment was started at t = 0, and CpG-2006 was added at 10 μmol/L after 24, 48, or 72 hours of IL-13 stimulation. All stimulations were maintained until the end of the experiment. Extended time exposure to IL-13 abolishes reactivity to CpG. u.s., Unstimulated. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E4 CpG-DNA enhances mRNA expression of TJs, claudin-1, claudin-4, claudin-7, and occludin, as well as that of the TJ-associated molecules ZO-1, ZO-2, and ZO-3. The dose-dependent response of mRNA expression of TJ-related molecules is shown. Bronchial epithelial cells from nonasthmatic subjects were treated with CpG-2006 (A). Control DNA did not show any effect (B). *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E5 Relative fluorescence signal intensity of claudin-4 and ZO-1 with or without CpG-2006 stimulation in bronchial epithelial cells from nonasthmatic (A) and asthmatic (B) subjects. Each value was calculated after removing background signal by using ImageJ software. Five different view fields were compared (n = 3). *P < .05 and **P < .01. Journal of Allergy and Clinical Immunology  , e8DOI: ( /j.jaci ) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "CpG-DNA enhances the tight junction integrity of the bronchial epithelial cell barrier  Terufumi Kubo, MD, PhD, Paulina Wawrzyniak, MSc, Hideaki Morita,"

Similar presentations

TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis  Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.

TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis  Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.
Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components  Stephen R. Reeves, MD, PhD, Tessa.

Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components  Stephen R. Reeves, MD, PhD, Tessa.
Early suppression of basophil activation during allergen-specific immunotherapy by histamine receptor 2  Natalija Novak, MD, Nihal Mete, MD, Caroline.

Early suppression of basophil activation during allergen-specific immunotherapy by histamine receptor 2  Natalija Novak, MD, Nihal Mete, MD, Caroline.
Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F

Barbara Stanic, PhD, Willem van de Veen, PhD, Oliver F
Defective calcium signaling and disrupted CD20–B-cell receptor dissociation in patients with common variable immunodeficiency disorders  Annick A.J.M.

Defective calcium signaling and disrupted CD20–B-cell receptor dissociation in patients with common variable immunodeficiency disorders  Annick A.J.M.
Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 
Mechanisms of IFN-γ–induced apoptosis of human skin keratinocytes in patients with atopic dermatitis  Ana Rebane, PhD, Maya Zimmermann, PhD, Alar Aab,

Mechanisms of IFN-γ–induced apoptosis of human skin keratinocytes in patients with atopic dermatitis  Ana Rebane, PhD, Maya Zimmermann, PhD, Alar Aab,
IL-13 and TH2 cytokine exposure triggers matrix metalloproteinase 7–mediated Fas ligand cleavage from bronchial epithelial cells  Samuel J. Wadsworth,

IL-13 and TH2 cytokine exposure triggers matrix metalloproteinase 7–mediated Fas ligand cleavage from bronchial epithelial cells  Samuel J. Wadsworth,
The broad spectrum of interepithelial junctions in skin and lung

The broad spectrum of interepithelial junctions in skin and lung
TNF-α–mediated bronchial barrier disruption and regulation by src-family kinase activation  Michelle A. Hardyman, PhD, Emily Wilkinson, BSc, Emma Martin,

TNF-α–mediated bronchial barrier disruption and regulation by src-family kinase activation  Michelle A. Hardyman, PhD, Emily Wilkinson, BSc, Emma Martin,
Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis  Eva Gros, MSc, Susanne Petzold,
Impaired barrier function in patients with house dust mite–induced allergic rhinitis is accompanied by decreased occludin and zonula occludens-1 expression 

Impaired barrier function in patients with house dust mite–induced allergic rhinitis is accompanied by decreased occludin and zonula occludens-1 expression 
Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids  Kittipong Maneechotesuwan, MD, PhD, Sirinya.

Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids  Kittipong Maneechotesuwan, MD, PhD, Sirinya.
Activation of protease-activated receptor 2 leads to impairment of keratinocyte tight junction integrity  Peter Nadeau, BS, Mason Henehan, BS, Anna De.

Activation of protease-activated receptor 2 leads to impairment of keratinocyte tight junction integrity  Peter Nadeau, BS, Mason Henehan, BS, Anna De.
Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,

Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,
IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,

IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,
Julie M. Caldwell, PhD, Carine Blanchard, PhD, Margaret H

Julie M. Caldwell, PhD, Carine Blanchard, PhD, Margaret H
T-cell regulation during viral and nonviral asthma exacerbations

T-cell regulation during viral and nonviral asthma exacerbations
FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells  Shigeru Okumura, DDS, PhD, Hironori Sagara,

FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells  Shigeru Okumura, DDS, PhD, Hironori Sagara,
Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion 

Similar presentations

Ads by Google