1. Quiz#10 LC710 10/27/10 name___________
Given the following transgenes co-existing in the same animal.
rtta binds DNA if Dox
is present
CMVp
rtta I.R.E.S GFP
tetO
TATA
RFP
Q1 0.5 pt (circle one) :
Q2 0.5 pt (circle one) :
Cellular Fluorescence observed
prior to addition of Doxycycline is:
Red
Green
Both
Neither
Cellular Fluorescence observed
after addition of Doxycycline is:
Red
Green
Both
Neither
Q3: 1pt What does tta do in the absence of Dox?________________________
What does tta do in the presence of Dox?________________________

2. the brain expressing Pax6 and/or Hox1.
Given the following transgenes co-existing in the same animal. 4 3 8
Pax6p 2 7 12
CreERt2 I.R.E.S GFP 11 1 6
Hox1p 5 10 9
loxP-RFP-loxP-TOXIN
Above are WT cells in
the brain expressing Pax6
and/or Hox1.
If expressed, TOXIN will Kill cells (disappear)
Q4 (2pts)
Q5 (2pts)
Prior to addition of TAM, which Cells are:
After TAM addition, which Cells are:
Red only:
Green only:
Both:
Neither:
Red only:
Green only:
Both:
Neither:
TAM= TAMOXIFEN
Q6 (2pts) : in the above transgene, loxP sites are in this orientation:
ATAACTTCGTATAATGTATGCTATACGAAGTTAT
= loxP sequence
This experiment is actually poorly designed and the loxP sites need to oriented as follows:
Hox1p
Why?