1. Management of bacterial (Ralstonia solanaceerum) wilt in tomato (Solanum lycopersicum L.) using resistant African eggplant (Solanum spp) rootstocks
T. NIKUZE, E. M. ATEKA, W. O. OWINO AND J. AMBUKO
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2. INTRODUCTION
Tomato is an economically important horticultural crop in Kenya
Progressive decline in tomato production
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3. Cont… Increased cost of production and reduction in household income
Constantly attacked by BW
Wide host range and widespread
Mitigated through use of resistant varieties
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4. Performance of tomato in Kenya 2012 – 2015
Year
Area (000) Ha
Volume (MT)
Value (000, Ksh)
2012 19
364
103
2013 21
384
116
2014 24
400
118
2015 25
410
120
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5. Objectives
To determine phenotypic reaction of African eggplant accessions to bacterial wilt infection
To identify resistant markers for bacterial wilt in different accessions of African eggplant
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6. Materials and Methods
1. To determine the phenotypic reaction of African eggplant accessions to bacterial wilt infection
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7. Bacterial wilt status of experimental field
Soil sample
Number of colonies
Population density (cfu/ml S1
177
1.6 x 107 S2
148
5.9 x 106 S3
154
9.4 x106
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8. Trans-Planting Eggplant seedlings Inoculation Field Greenhouse
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9. Results
Healthy plant (A), bacterial wilt symptoms (B, C and D), E, F and G BW oozing from plant sections a D b B c
Oozy stem F E
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10. Cont…
Morphological characteristics on TZC (Kelman) media
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11. Reaction trends
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12. Trends cont…
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13. Trends cont…
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14. Trends cont…
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15. Trends cont…
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16. Materials and methods
2. To identify resistant genes for bacterial wilt in different accessions of African eggplant
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17. DNA-extraction
CTAB
PCR
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18. Results
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19. Cont…..
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20. A1 A2 A A3 B1 B B3
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21. Discussion and Conclusion
There was difference in response to infection
Various mortality at different time point
No wilting symptoms in Accession RV and resistant markers
Accession RV did not react to BW and carried 5 SSR markers
Morphological characteristics of Ralstonia after isolation
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22. Cont… Resistant markers were present in thirty accessions
There is association between accessions and origin
S. aethiopicum and S. anguivi good source of resistant genes
Resistant markers will help breeders to develop resistant tomato varieties to BW
Resistant accession can be used as rootstocks in management of BW
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23. References
Bi-hao, C. Jian-jun, L., Yong, W. and Guo-ju, C Inheritance and identification of SCAR marker linked to bacterial wilt-resistance in eggplant. African Journal of Biotechnology , 8(20), pp
Caguiat, X.G.I. and Hautea, D.M Genetic Diversity analysis of eggplant (solanum melongena l.) and related wild species in the philippines using morphological and ssr markers. sabrao Journal or Breeding and Genetics, 46(2), pp
Castillo, J. A. and Greenberg, J. T Evolutionary Dynamics of Ralstonia solanacearum." Applied and Environmental Micribiology, 73(4), pp
Lebeau, A., Gouy, M., Daunay, M. C., Wicker, E., Chiroleu, F., Prior, P., Frary, A. and Dintinger, J Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant. Theor Appl Genet, Volume 126, pp
Muthoni, J., Shimelis, H. and Melis, R Management of Bacterial Wilt [Rhalstonia solanacearum Yabuuchi et al., 1995] of Potatoes: Opportunity for Host Resistance in Kenya. Journal of Agricultural Science, 4(9), pp
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24. THANK YOU
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