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CobNST Cbr(II) HBA a,c-diamide CobN-ChlID CobB ? Hydrogenobyrinic acid salvage Cbr(I) L-thr HBA Btu? HBA BtuR Adenosyl-cobalamin precursor salvage CobO PduX Auxotroph Marinobacter HL-58 Cbr Btu AdoCbr L-thr-3P Prototrophs with Btu transporter Phormidium OSCR (PsB12) Halomonas HL-48 & HL-93 Rhizobiales HL-109 CbiP CobQ CobD CobC b-axial ligand attachment Cby Btu CobO AdoCby AP-P FMNH2 Auxotrophs with Btu transporter Roseibaca HL-91 (Cbr) Rhodo bin09, bin18 (Cbr) Rhodo bin07, bin12 (Cby ) Erythrobacter HL-111 (Cbi-GDP) BtuR PsB12 Btu Ado-PsB12 CbiZ PduO CbiB CobD BluB Cbi Btu AdoCbi CobU AdoCbi-P DMB CobU CobP CobT CobU Cbi-GDP Btu AdoCbi-GDP a-ribazole-5P Auxotrophs with Btu transporter Bacteroidetes bin01 Algoriphagus HL-49 Marinobacter HL-55 Porphyrobacter HL-46 Oceanicaulis bin04 Rhodo bin08 CobS CobV Supplementary Figure S6 | Corrinoid salvage and biosynthesis. A portion of adenosylcobalamin biosynthetic pathway that includes precursors likely to be salvaged by UCC members includes metabolites shown in white rectangles: HBA, hydrogenobyrinic acid; Cbr(II), cob(II)yrinate a,c-diamide; Cbr(I), cob(I)yrinate a,c-diamide; AdoCbr, adenosylcobyrinate a,c-diamide; AcoCby, adenoyslcobyrate; AdoCbi-P, adenosylcobinamide phosphate; AdoCbi-GDP, adenosylcobinamide-guanosine diphosphate; AdoCbl-P, adenosylcobalamine-5-phosphate; AdoCbl, adenosylcobalamine; L-thr, L-threonine; L-thr-3P, L-threonine-3-phosphate; AP-P, (R)-1-amino-2-propanol O-2-phosphate; FMNH2, reduced flavin mononucleotide; DMB, 5,6-dimethylbenzimidazole; a-ribazole-5P, a-5,6-dimethylbenzimidazole-ribosyl-5’-phosphate; PsB12, pseudo-cobalamin B12; Ado-PsB12, adenosyl-cobalamin B12; Cbr, cobyrinic acid; Cby, cobyric acid; AdoCbi, adenosylcobinamide; Cbi, cobinamide; P, phosphate. Btu refers to the ABC-type corrinoid transporter and BtuB TonB-dependent outer membrane corrinoid receptor which is found in most members. Enzymes involved biosynthesis appear in blue rectangles and those in salvage in light red rectangles: CobB, glutamine-hydrolysing hydrogenobyrinic acid a,c-diamide synthase; CobNST and CobN-ChlD, alternative cobaltochelatase complexes; BtuR and PduO, alternative corrinoid adenosyltransferases; CbiP, cobyric acid synthase; PduX, L-threonine kinase; CobD, L-threonine 3-O-phosphate decarboxylase; CbiB, adenosylcobinamide-phosphate synthase; CobU, bifunctional adenosylcobinamide kinase / adenosylcobinamide-phosphate guanylyltransferase; CobT, nicotinate mononucleotide--dimethylbenzimidazole phosphoribosyltransferase; BluB, 5,6-dimethylbenzimidazole synthase; CobS, adenosylcobinamide-GDP ribazoletransferase; CobC and CblXY, alternative alpha-ribazole-5'-phosphate phosphatases; CbiZ, adenosylcobinamide hydrolase. Non-orthologous replacements are indicated by filled blue circles. Red arrows indicate pathways for recycling and salvage of adenosylcobalamin precursors. Common alternative enzyme names appear next to blue rectangles. Note that this figure includes corrections to the last steps of biosynthesis that have been previously reported46 but infrequently reflected in recent publications. a-axial ligand attachment Cobalamin salvage AdoCbl-P CobO CobC CblXY BtuR Cbl cyanocobalamin Btu AdoCbl PduO
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