1. CobNST
Cbr(II)
HBA a,c-diamide
CobN-ChlID
CobB ?
Hydrogenobyrinic acid salvage
Cbr(I)
L-thr
HBA
Btu?
HBA
BtuR
Adenosyl-cobalamin precursor salvage
CobO
PduX
Auxotroph
Marinobacter HL-58
Cbr
Btu
AdoCbr
L-thr-3P
Prototrophs with Btu transporter
Phormidium OSCR (PsB12)
Halomonas HL-48 & HL-93
Rhizobiales HL-109
CbiP
CobQ
CobD
CobC
b-axial ligand attachment
Cby
Btu
CobO
AdoCby
AP-P
FMNH2
Auxotrophs with Btu transporter
Roseibaca HL-91 (Cbr)
Rhodo bin09, bin18 (Cbr)
Rhodo bin07, bin12 (Cby )
Erythrobacter HL-111 (Cbi-GDP)
BtuR
PsB12
Btu
Ado-PsB12
CbiZ
PduO
CbiB
CobD
BluB
Cbi
Btu
AdoCbi
CobU
AdoCbi-P
DMB
CobU
CobP
CobT
CobU
Cbi-GDP
Btu
AdoCbi-GDP
a-ribazole-5P
Auxotrophs with Btu transporter
Bacteroidetes bin01
Algoriphagus HL-49
Marinobacter HL-55
Porphyrobacter HL-46
Oceanicaulis bin04
Rhodo bin08
CobS
CobV
Supplementary Figure S6 | Corrinoid salvage and biosynthesis. A portion of adenosylcobalamin biosynthetic pathway that includes precursors likely to be salvaged by UCC members includes metabolites shown in white rectangles: HBA, hydrogenobyrinic acid; Cbr(II), cob(II)yrinate a,c-diamide; Cbr(I), cob(I)yrinate a,c-diamide; AdoCbr, adenosylcobyrinate a,c-diamide; AcoCby, adenoyslcobyrate; AdoCbi-P, adenosylcobinamide phosphate; AdoCbi-GDP, adenosylcobinamide-guanosine diphosphate; AdoCbl-P, adenosylcobalamine-5-phosphate; AdoCbl, adenosylcobalamine; L-thr, L-threonine; L-thr-3P, L-threonine-3-phosphate; AP-P, (R)-1-amino-2-propanol O-2-phosphate; FMNH2, reduced flavin mononucleotide; DMB, 5,6-dimethylbenzimidazole; a-ribazole-5P, a-5,6-dimethylbenzimidazole-ribosyl-5’-phosphate; PsB12, pseudo-cobalamin B12; Ado-PsB12, adenosyl-cobalamin B12; Cbr, cobyrinic acid; Cby, cobyric acid; AdoCbi, adenosylcobinamide; Cbi, cobinamide; P, phosphate. Btu refers to the ABC-type corrinoid transporter and BtuB TonB-dependent outer membrane corrinoid receptor which is found in most members. Enzymes involved biosynthesis appear in blue rectangles and those in salvage in light red rectangles: CobB, glutamine-hydrolysing hydrogenobyrinic acid a,c-diamide synthase; CobNST and CobN-ChlD, alternative cobaltochelatase complexes; BtuR and PduO, alternative corrinoid adenosyltransferases; CbiP, cobyric acid synthase; PduX, L-threonine kinase; CobD, L-threonine 3-O-phosphate decarboxylase; CbiB, adenosylcobinamide-phosphate synthase; CobU, bifunctional adenosylcobinamide kinase / adenosylcobinamide-phosphate guanylyltransferase; CobT, nicotinate mononucleotide--dimethylbenzimidazole phosphoribosyltransferase; BluB, 5,6-dimethylbenzimidazole synthase; CobS, adenosylcobinamide-GDP ribazoletransferase; CobC and CblXY, alternative alpha-ribazole-5'-phosphate phosphatases; CbiZ, adenosylcobinamide hydrolase. Non-orthologous replacements are indicated by filled blue circles. Red arrows indicate pathways for recycling and salvage of adenosylcobalamin precursors. Common alternative enzyme names appear next to blue rectangles. Note that this figure includes corrections to the last steps of biosynthesis that have been previously reported46 but infrequently reflected in recent publications.
a-axial ligand attachment
Cobalamin salvage
AdoCbl-P
CobO
CobC
CblXY
BtuR
Cbl
cyanocobalamin
Btu
AdoCbl
PduO