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Increased ALK Gene Copy Number and Amplification are Frequent in Non-small Cell Lung Cancer  Marta Salido, MSc, Lara Pijuan, MD, PhD, Luz Martínez-Avilés,

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Presentation on theme: "Increased ALK Gene Copy Number and Amplification are Frequent in Non-small Cell Lung Cancer  Marta Salido, MSc, Lara Pijuan, MD, PhD, Luz Martínez-Avilés,"— Presentation transcript:

1 Increased ALK Gene Copy Number and Amplification are Frequent in Non-small Cell Lung Cancer 
Marta Salido, MSc, Lara Pijuan, MD, PhD, Luz Martínez-Avilés, MSc, Ana B. Galván, MSc, Israel Cañadas, MSc, Ana Rovira, PhD, Montserrat Zanui, MD, Alejandro Martínez, MD, Raquel Longarón, Francisco Sole, PhD, Sergio Serrano, MD, PhD, Beatriz Bellosillo, PhD, Murry W. Wynes, PhD, Joan Albanell, MD, PhD, Fred R. Hirsch, MD, PhD, Edurne Arriola, MD, PhD  Journal of Thoracic Oncology  Volume 6, Issue 1, Pages (January 2011) DOI: /JTO.0b013e3181fb7cd6 Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

2 FIGURE 1 A, Left (arrows): atypical anaplastic lymphoma kinase (ALK) rearrangement with deletion of 5′ region of ALK (loss of green-labeled probe centromeric to ALK). Right: normal echinoderm microtubule-associated protein-like 4 (EML4) fusion pattern. B, Left (arrows): typical gene breakage signal of ALK (yellow wild-type allele, and single red and green probe signals for the rearranged allele). Right (arrows): EML4 typical probe breakage. Journal of Thoracic Oncology 2011 6, 21-27DOI: ( /JTO.0b013e3181fb7cd6) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

3 FIGURE 2 A, Anaplastic lymphoma kinase (ALK) amplification (orange/green fusion signals) in a cell with three copies of CEP2 (red signals). B, Polysomic cells from the same amplified case showing 3 to 4 copies of ALK and 3 to 4 copies of the chromosome 2 centromere. Journal of Thoracic Oncology 2011 6, 21-27DOI: ( /JTO.0b013e3181fb7cd6) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

4 FIGURE 3 Immunohistochemical staining analysis of anaplastic lymphoma kinase (ALK) protein expression. Pictures are at ×40 magnification. A, Case with ALK overexpression H score: 260. B, Case with ALK overexpression H score: 80. Both cases correspond to the tumors from patients with echinoderm microtubule-associated protein-like 4 (EML4)-ALK translocations by fluorescence in situ hybridization (FISH). Journal of Thoracic Oncology 2011 6, 21-27DOI: ( /JTO.0b013e3181fb7cd6) Copyright © 2011 International Association for the Study of Lung Cancer Terms and Conditions

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