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Melatonin and structurally similar compounds have differing effects on inflammation and mitochondrial function in endothelial cells under conditions mimicking sepsis D.A. Lowes, A.M. Almawash, N.R. Webster, V.L. Reid, H.F. Galley British Journal of Anaesthesia Volume 107, Issue 2, Pages (August 2011) DOI: /bja/aer149 Copyright © 2011 The Author(s) Terms and Conditions
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Fig 1 NFκB in nuclear extracts of endothelial cells treated for 4 h with 2 µg ml−1 LPS plus 20 µg ml−1 PepG plus either (a) melatonin, (b) 6-hydroxymelatonin, (c) tryptamine, or (d) indole-3-carboxylic acid. Box and whisker plots show median, IQR, and full range (n=6). P-value is Kruskal–Wallis. *Significantly different from untreated cells (P<0.05); #significantly different from cells treated with LPS/PepG only (P<0.05). British Journal of Anaesthesia , DOI: ( /bja/aer149) Copyright © 2011 The Author(s) Terms and Conditions
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Fig 2 IL-6 in culture medium from endothelial cells treated for 24 h with 2 µg ml−1 LPS plus 20 µg ml−1 PepG plus either (a) melatonin, (b) 6-hydroxymelatonin, (c) tryptamine, or (d) indole-3-carboxylic acid. Box and whisker plots show median, IQR, and full range (n=6). P-value is Kruskal–Wallis. *Significantly different from untreated cells (P<0.05); #significantly different from cells treated with LPS/PepG only (P<0.05). British Journal of Anaesthesia , DOI: ( /bja/aer149) Copyright © 2011 The Author(s) Terms and Conditions
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Fig 3 IL-8 in culture medium from endothelial cells treated for 24 h with 2 µg ml−1 LPS plus 20 µg ml−1 PepG plus either (a) melatonin, (b) 6-hydroxymelatonin, (c) tryptamine, or (d) indole-3-carboxylic acid. Box and whisker plots show median, IQR, and full range (n=6). P-value is Kruskal–Wallis. *Significantly different from untreated cells (P<0.05); #significantly different from cells treated with LPS/PepG only (P<0.05). British Journal of Anaesthesia , DOI: ( /bja/aer149) Copyright © 2011 The Author(s) Terms and Conditions
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Fig 4 Total glutathione in endothelial cells treated for 7 days with 2 µg ml−1 LPS plus 20 µg ml−1 PepG plus either (a) melatonin, (b) 6-hydroxymelatonin, (c) tryptamine, or (d) indole-3-carboxylic acid. Box and whisker plots show median, IQR, and full range (n=6). P-value is Kruskal–Wallis. *Significantly different from untreated cells (P<0.05); #significantly different from cells treated with LPS/PepG only (P<0.05). British Journal of Anaesthesia , DOI: ( /bja/aer149) Copyright © 2011 The Author(s) Terms and Conditions
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Fig 5 JC-1 fluorescence ratio as a marker of mitochondrial membrane potential in endothelial cells treated for 7 days with 2 µg ml−1 LPS plus 20 µg ml−1 PepG plus either (a) melatonin, (b) 6-hydroxymelatonin, (c) tryptamine, or (d) indole-3-carboxylic acid. Box and whisker plots show median, IQR, and full range (n=6). P-value is Kruskal–Wallis. *Significantly different from untreated cells (P<0.05); #significantly different from cells treated with LPS/PepG only (P<0.05). British Journal of Anaesthesia , DOI: ( /bja/aer149) Copyright © 2011 The Author(s) Terms and Conditions
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Fig 6 Metabolic activity measured using Alamar Blue in endothelial cells treated for 7 days with 2 µg ml−1 LPS plus 20 µg ml−1 PepG plus either (a) melatonin, (b) 6-hydroxymelatonin, (c) tryptamine, or (d) indole-3-carboxylic acid. Box and whisker plots show median, IQR, and full range (n=6). P-value is Kruskal–Wallis. *Significantly different from untreated cells (P<0.05); #significantly different from cells treated with LPS/PepG only (P<0.05). British Journal of Anaesthesia , DOI: ( /bja/aer149) Copyright © 2011 The Author(s) Terms and Conditions
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