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A technique for more precise distinction between catagen and telogen human hair follicles ex vivo  Irene Hernandez, PhD, Majid Alam, PhD, Christopher.

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Presentation on theme: "A technique for more precise distinction between catagen and telogen human hair follicles ex vivo  Irene Hernandez, PhD, Majid Alam, PhD, Christopher."— Presentation transcript:

1 A technique for more precise distinction between catagen and telogen human hair follicles ex vivo 
Irene Hernandez, PhD, Majid Alam, PhD, Christopher Platt, PhD, Jonathan Hardman, PhD, Eleanor Smart, MSc, Enrique Poblet, MD, Marta Bertolini, PhD, Ralf Paus, MD, Francisco Jimenez, MD  Journal of the American Academy of Dermatology  Volume 79, Issue 3, Pages (September 2018) DOI: /j.jaad Copyright © 2018 American Academy of Dermatology, Inc. Terms and Conditions

2 Fig 1 Macroscopic analysis of hair follicles isolated from follicular units is more definitive after methylene blue staining. A, Hair follicle in late catagen stage, with the epithelial strand (arrow) clearly visible after methylene blue (0.02%) staining. B, Nonstained hair follicle that cannot be clearly identified under the stereomicroscope as either late catagen or telogen, but after methylene blue staining (C), the small remaining epithelial strand (dotted-line) allows us to identify it as late catagen. D, Ki67 staining and TUNEL confirms this strand is a late catagen hair follicle, with the presence of several apoptotic, TUNEL positive cells (arrowheads). TUNEL, Terminal deoxynucleotidyl transferase dUTP nick-end labeling. Journal of the American Academy of Dermatology  , DOI: ( /j.jaad ) Copyright © 2018 American Academy of Dermatology, Inc. Terms and Conditions

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